International Archives of Medical Sciences and Public Health
Vol. 7 No. 1 (2026): International Archives of Medical Sciences and Public Health

ANTIBACTERIAL ACTIVITY OF COMBINED ETHANOL EXTRACTS OF CLITORIA TERNATEA FLOWERS AND JATROPHA GOSSYPIFOLIA LEAVES AGAINST ESCHERICHIA COLI

Sabariah Sabariah (Faculty of Medicine, Universitas Islam Al-Azhar, Mataram, Indonesia)
Sabrina Intan Zoraya (Faculty of Medicine, Universitas Islam Al-Azhar, Mataram, Indonesia)
Dinda Ayuni Rusma Putri (Faculty of Medicine, Universitas Islam Al-Azhar, Mataram, Indonesia)



Article Info

Publish Date
01 Jul 2026

Abstract

The growing resistance of Escherichia coli to antibiotics has increased interest in plant-derived antibacterial agents. This study evaluated the antibacterial activity of combined ethanol extracts of Clitoria ternatea flowers and Jatropha gossypifolia leaves against E. coli. An in vitro experimental study with a post-test-only design was conducted. Extracts were prepared by maceration using 96% ethanol and subjected to phytochemical screening. Antibacterial activity was assessed using the disk diffusion method at concentrations of 20%, 40%, 60%, 80%, and 100%, with ciprofloxacin and dimethyl sulfoxide (DMSO) as controls. Each treatment was tested in four replicates. Phytochemical screening identified alkaloids, flavonoids, tannins, phenolic/hydroquinone compounds, and steroids. The largest inhibition zone was observed at 20% (12.25mm), followed by 40% (10.25 mm), 60% (8.00 mm), and 80% (6.25mm), while no inhibition occurred at 100%. Significant differences were found among groups (p < 0.001). These findings indicate measurable but limited antibacterial activity against E. coli.

Copyrights © 2026






Journal Info

Abbrev

iamsph

Publisher

Subject

Health Professions Medicine & Pharmacology Nursing Public Health

Description

International Archives of Medical Sciences and Public Health is a peer-reviewed journal dedicated to disseminating and discussing scientific literature and other health-related research. This journal is intended to serve as a forum for communication between stakeholders in health research, including ...