This study addressed the absence of assessment instruments that simultaneously capture the integration of technology, pedagogy, and content with sustainability-oriented competencies in science teacher education. It aimed to develop and validate an assessment rubric that integrates technological pedagogical content knowledge (TPACK) with education for sustainable development (ESD) for pre-service science teachers. The rubric was developed through an iterative research and development process involving literature-based construct mapping, expert review, and quantitative content validation. Three expert validators evaluated 20 rubric items, and Aiken’s V was used to quantify content validity. The results showed item-level Aiken’s V values ranging from 0.78 to 1.00, with 18 items in the very high category and 2 items in the high category, and an overall V of 0.96, indicating excellent expert agreement. These findings demonstrate that the rubric provides a valid and conceptually robust tool for assessing pre-service science teachers’ ability to design technology-enhanced, sustainability-oriented science lessons. The rubric can support formative and summative assessment in teacher education programs and offers a foundation for future empirical and comparative studies on TPACK–ESD competence
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