Background: Neonatal asphyxia and iron-deficiency anemia are two leading causes of newborn morbidity and mortality. Delayed umbilical cord clamping (DCC) is an evidence-based intervention with the potential to prevent both conditions by optimizing placental transfusion following delivery. Objective: To analyze the effect of DCC duration (2 minutes, 3 minutes, and 7 minutes) on hemoglobin levels and oxygen saturation in term neonates. Methods: This study used a quasi-experimental design with a nonequivalent control group design. The sample consisted of 30 term neonates divided into three groups (n = 10 per group): the 2-minute, 3-minute, and 7-minute delay groups. Data were analyzed using the One-Way ANOVA test and Tukey HSD post hoc test. Results: There were significant differences in hemoglobin levels (F = 43.471; p = 0.001) and oxygen saturation (F = 8.520; p = 0.001) between groups. The highest mean hemoglobin level was found in the 7-minute delay group (23.230 ± 0.807 g/dL), followed by the 3-minute group (20.240 ± 2.558 g/dL), and the 2-minute group (15.340 ± 5.277 g/dL). Post hoc testing revealed significant differences between all hemoglobin group pairs. Regarding oxygen saturation, the 3-minute and 7-minute delay groups showed no significant difference (p = 0.484), indicating that the effectiveness threshold was reached at the third minute Conclusion: DCC significantly improves hemoglobin levels and oxygen saturation in term neonates. A minimum delay of 3 minutes recommended as the optimal duration in newborn care as an integrated strategy for preventing neonatal asphyxia and anemia.
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