<![CDATA[This study utilizes green nanotechnology by employing Trema orientalis leaf extract (TO-EE) as a natural bioreductant to synthesize silver nanoparticles (AgNPs-TO) and apply them in biopolymer film-based food packaging. AgNPs were synthesized using a stirrer method and characterized by UV-Vis, FTIR, XRD, PSA, and TEM. UV-Vis confirmed nanoparticle formation with a λmax at 430 nm (SPR). FTIR indicated phenolic and carbonyl groups in the reduction of Ag+ to Ag0. XRD showed a face-centered cubic silver structure with minor AgCl presence. PSA revealed an average size of 56.46 nm with high polydispersity (>0.5), while TEM showed spherical, heterogeneous particles. Antioxidant activity (DPPH) showed IC50 values of 128.40 µg/mL (AgNPs-TO) and 75.59 µg/mL (TO-EE). Anti-photooxidation tests in a linoleic acid system demonstrated that AgNPs-TO and BP/AgNPs-TO films suppressed hydroperoxide formation. Preliminary BSLT screening showed an LC50 <1000 ppm, and 5 ppm AgNPs were selected for incorporation into the biopolymer film. The additional AgNPs-TO also affects the physical properties of the biopolymer film. These results indicate that AgNPs-TO are promising as active agents in environmentally friendly food packaging.]]>
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