Increasing feed costs for KUB chickens encourage the development of alternative feed sources that are easy to cultivate and sustainable. This study aimed to describe the quality of liquid organic fertilizer (LOF) made from rice-washing wastewater fermented with local microorganisms (MOL) derived from spoiled rice, as well as its effect on the biomass growth of Azolla microphylla as an alternative green feed. The research methods included testing the LOF quality based on pH and nutrient content parameters in accordance with the Regulation of the Minister of Agriculture No. 70/2011, as well as conducting an Azolla cultivation experiment with four treatments: P0 (0 mL/10 L), P1 (10 mL/10 L), P2 (25 mL/10 L), and P3 (35 mL/10 L), each with three replications. Environmental parameters measured included pH, temperature, and light intensity, while Azolla biomass was measured weekly. Data were analyzed descriptively and quantitatively using mean values and standard deviation. The results showed that the LOF contained N 0.33%, P₂O₅ 0.229%, K₂O 0.29%, organic C 1.61%, C/N ratio 4.88, with pH 4 and Fe 8.30 mg/kg in comparison with the quality standards. Treatment P1 produced the highest biomass of 83.33 g in the fourth week. This study indicates that rice-washing wastewater-based LOF is able to increase the biomass of Azolla microphylla and support SDGs 12.5.
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