Catfish are highly tolerant of changes in water quality, making them easier to cultivate. Catfish are omnivorous, consuming fish bones and catfish maggots. One way to substitute catfish feed is by adding chicken manure, made with other feed additives. This study used four treatments for chicken manure substitute feed: A1 (without MA), A2 (10% MA), A3 (15% MA), and A4 (20% MA). Chicken manure was fed 3 times a day and maintained for one month. Water changes were carried out once a week by reducing the water by 50%. Water quality testing was carried out twice, namely at the beginning and end of maintenance. Water quality sampling was carried out in fish pond conditions that had not been reduced. Water quality tests carried out were water physics tests, water chemistry tests and water biology tests which included temperature, nitrate, phosphate, ammonia, pH, DO, TSS and e-coli bacteria. The results of physical water quality obtained during maintenance were water temperature in each treatment had a relative range of 27-29oC at the beginning and end of maintenance. The results of chemical quality at the beginning and end were nitrite obtained in the range of 0.01-0.03 mg/l, phosphate range of 2.10-3.2 mg/l, DO range of 1.82-3.91 mg/l, ammonia range of 3.06-3.67 mg/l, TSS range of 0.20-0.60, pH range of 6.10-6.70. The biological water quality results obtained were e-coli values ranging from 90-130 CFU/ml. The water quality results obtained were still within the normal range for catfish cultivation and the water quality values obtained were compared with PP No. 82 2001, SNI Catfish Breeding, KMN Environmental No. 115 of 2003. Key words: water quality, catfish, feed substitution, chicken manure
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