EFFECTS OF DIETARY SALT AND HERBAL SUPPLEMENTATION ON HYDROLYTIC BACTERIAL COMMUNITIES IN THE FILTRATION SYSTEM OF BONYLIP BARB (Osteochilus vittatus)

Siti Khoirun Nisa (Magister Program in Aquatic Resources, Fisheries Department, Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman, Jl. Dr Soeparno, Komplek GOR Soesilo Soedarman, Karangwangkal, Purwokerto Utara, Banyumas, Jawa Tengah 53122)
Syafrie Alfauzie (Aquaculture Study Program, Fisheries Department, Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman, Jl. Dr Soeparno, Komplek GOR Soesilo Soedarman, Karangwangkal, Purwokerto Utara, Banyumas, Jawa Tengah 53122)
Mevira Sekar Chaerunissa (Aquaculture Study Program, Fisheries Department, Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman, Jl. Dr Soeparno, Komplek GOR Soesilo Soedarman, Karangwangkal, Purwokerto Utara, Banyumas, Jawa Tengah 53122)
Hamdan Syakuri (Program Studi Akuakultur, Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Jenderal Soedirman)
Taufik Budhi Pramono (Magister Program in Aquatic Resources, Fisheries Department, Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman, Jl. Dr Soeparno, Komplek GOR Soesilo Soedarman, Karangwangkal, Purwokerto Utara, Banyumas, Jawa Tengah 53122)
Dewi Nugrayani (Aquaculture Study Program, Fisheries Department, Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman, Jl. Dr Soeparno, Komplek GOR Soesilo Soedarman, Karangwangkal, Purwokerto Utara, Banyumas, Jawa Tengah 53122)
Emyliana Listiowati (Aquaculture Study Program, Fisheries Department, Faculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman, Jl. Dr Soeparno, Komplek GOR Soesilo Soedarman, Karangwangkal, Purwokerto Utara, Banyumas, Jawa Tengah 53122)

Jurnal Perikanan
Vol 15 No 5 (2025): JURNAL PERIKANAN

Dietary supplementation with salt and herbal additives may influence not only the gut microbiota of cultured fish but also the bacterial communities in the surrounding water, including filtration systems. This study aimed to characterize the morphotypes and hydrolytic activity of bacteria isolated from the filtration system of Bonylip barb (Osteochilus vittatus) fed diets supplemented with salt and herbal concoctions. A completely randomized design (CRD) was employed with four treatments and three replicates: P1) Control; P2) 3% salt supplementation; P3) 10 mL/kg feed herbal supplementation; and P4) combined 3% salt and 10 mL/kg feed herbal supplementation. After 60 days of culture, bacterial isolates from the filtration system were assessed for colony morphotypes and hydrolytic activity, including proteolytic, amylolytic, cellulolytic, and lipolytic indices. Results indicated that all supplemented treatments (P2–P4) yielded a higher diversity of bacterial morphotypes compared to the control. Notably, proteolytic activity was significantly elevated in the salt-supplemented group (P2) relative to both the control and herbal-only treatments. However, salt and herbal supplementation did not significantly affect amylolytic, cellulolytic, or lipolytic activity indices. These findings suggest that dietary salt influences the functional composition of hydrolytic bacteria in aquaculture filtration systems.