SITAWA : Jurnal Farmasi Sains dan Obat Tradisional
Vol. 3 No. 2 (2024): SITAWA : Jurnal Farmasi Sains dan Obat Tradisional

DETERMINATION OF TOTAL PHENOL CONTENT IN METHANOL EXTRACT OF ROSEMARY (Rosmarinus officinalis L.) LEAVES BY DIGETION METHODS USING UV-VIS SPECTROFOTOMETRY METHODS

Mercyska Mercyska Suryandari (Akademi Farmasi Surabaya)
Risma Dwi Rahayu (Akademi Farmasi Surabaya, Surabaya, Jawa Timur)
Noor Syafika Rani (Akademi Farmasi Surabaya, Surabaya, Jawa Timur)



Article Info

Publish Date
31 Jul 2024

Abstract

The methanol extract of resemary leaves contains terpenoids, alkanoids, flavonoids, tannins, steroids, saponins and polyphenols. The aim of this research was to determine the total phenol of methanol extract of rosemary (Rosmarinus officinalis L.) by digestion method using the UV-Vis spectrophotometric method. In this study, methanol extract of rosemary leaves extracted using the digestion method was used as a sample and gallic acid as a standard. A standard solution of 200 ppm gallic acid was prepared to determine the operating time at 100 minutes and the maximum wavelength at 747 nm. Next, a comparison solution of gallic acid was made with a concentration range of 100, 125, 150, 175, 200 ppm and produced a linear regression equation, namely y = 0.0039× - 0.1146 with a correlation coefficient (r) of 0.9842. Determination of total phenol content was replicated 3 times and the absorption value was observed. The research results showed that the average total phenol content was 57.0317 mgGAE/g extract.

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Journal Info

Abbrev

sitawa

Publisher

Subject

Description

SITAWA: Jurnal Farmasi Sains dan Obat Tradisional merupakan jurnal ilmiah nasional akses terbuka yang dikelola oleh Akademi Farmasi Imam Bonjol Bukittinggi dan terbit dua kali setahun pada bulan Januari dan Juli, yang mempublikasikan hasil penelitian unggulan di bidang Farmasi, Teknologi dan Desain ...