Chrysanthemum is an ornamental plant prepared for export by the City of Tomohon, but the availability of seedlings remains limited. Vegetative propagation with the aid of garlic extract as a natural growth regulator (PGR) can be a solution to accelerate the rooting of shoot cuttings.This study aimed to determine the effect of using garlic extract (Allium sativum L.) and to identify the best concentration of garlic extract as a natural PGR on the root development of chrysanthemum shoot cuttings (Chrysanthemum morifolium R.). The research was conducted in September 2024 at the Screen House of the UPTD Seed Breeding and Agrotourism Show Window, Tomohon City. The study used a Completely Randomized Design (CRD) with five levels of garlic extract concentration treatments: P0 (0% garlic extract + 100% sterile water), P1 (25% garlic extract + 75% sterile water), P2 (50% garlic extract + 50% sterile water), P3 (75% garlic extract + 25% sterile water), P4 (100% garlic extract + 0% sterile water). Each treatment was replicated three times, with three cuttings per experimental unit, resulting in a total of 45 cuttings. The observation data were analyzed using ANOVA and further tested with the 5% Least Significant Difference (LSD) test. The results showed that garlic extract had an effect on the root length of chrysanthemum shoot cuttings and provided protection against pest and disease attacks. Keywords: Garlic extract; Natural PGR; Shoot cuttings; Root growth; Chrysanthemum.
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