This research aims to analyze the application of mise-en-scène on facial gesture of animated characters in Public Service Advertisement (PSA) "Sasa and Wiwit" produced by the Palm Oil Plantation Fund Management Agency (BPDPKS). The focus of the research is how the characters' facial expressions support the delivery of messages about the benefits of palm oil. The research used a qualitative method with data in the form of screenshots of scenes featuring facial expressions. The analysis was conducted using a mise-en-scène approach that pays attention to visual elements such as eyes, eyebrows, lips, and head shape. Allan Pease's nonverbal communication theory and Paul Ekman's basic emotion expression theory were used as the basis for interpreting the meaning of facial expressions. The results show that Sasa and Wiwit's facial expressions highlight positive emotions such as happiness, friendliness, and openness. Elements such as open round eyes, subtly arched eyebrows, and smiling lips on Sasa and open curved lips on Wiwit are able to build an emotional connection with the audience. The shape of the head resembling a palm kernel strengthens the visual identity of the ad. This finding confirms the importance of facial expression design in reinforcing the social message of animation. This research contributes to the study of mise-en-scène in non-commercial animation and serves as a practical guide for PSA makers. The limitation of the research is that it only analyzes one scene, so further studies are needed with a wider scope of objects and visual elements. Keywords: Animation, Facial Gesture, Palm Oil
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