Work fatigue is a common occupational health and safety (OHS) issue among healthcare workers. It may reduce work productivity and service quality while increasing the likelihood of work-related errors. High service demands, limited healthcare personnel, and excessive workloads may further contribute to the occurrence of fatigue. This study aimed to identify factors associated with work fatigue among healthcare workers at Public Health Centers in Pondok Aren District. A quantitative analytical study with a cross-sectional design was conducted involving 187 healthcare workers selected through total sampling. The independent variables included age, sex, educational level, length of employment, workload, and exercise habits, while work fatigue served as the dependent variable. Data were analyzed using univariate analysis, bivariate analysis with the chi-square test, and multivariate analysis through logistic regression. The findings indicated that 114 respondents (61%) experienced moderate work fatigue, while 73 respondents (39%) experienced high work fatigue. Significant associations were found between fatigue and length of employment, workload, and exercise habits. Multivariate analysis identified workload as the most dominant factor associated with work fatigue. The Nagelkerke R Square value of 0.324 indicated that the model explained 32.4% of the variation in work fatigue.
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