Dengue Hemorrhagic Fever (DHF) is a disease transmitted by the Aedes aegypti and Aedes albopictus mosquitoes. Repeated use of chemical larvicides can lead to vector resistance, environmental pollution, and the death of non-target organisms. To determine the effectiveness of papaya (Carica papaya L.) peel extract as a natural larvicide against instar III Aedes aegypti larvae. The research used a True Experimental design with a purposive random sampling method. The probit analysis results showed LC₅₀ values of 7.046% (3 hours), 4.976% (6 hours), and 3.196% (9 hours), while LT₅₀ values were 12.950 hours (1%), 9.386 hours (3%), and 6.073 hours (5%). Papaya peel extract has been proven to be effective as a natural larvicide against instar III Aedes aegypti larvae.
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