Jurnal Medik Veteriner
Vol. 9 No. 2 (2026): October

Effects of bacteriophage concentration and contact time on Enterobacteriaceae reduction in chicken drumsticks inoculated with Salmonella Typhimurium

Herwin Pisestyani (Laboratory of Veterinary Public Health, Division of Veterinary Public Health and Epidemiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia)
Denny Widaya Lukman (Laboratory of Veterinary Public Health, Division of Veterinary Public Health and Epidemiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia)
Havan Yusuf (Laboratory of Epidemiology, Division of Veterinary Public Health and Epidemiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia)
Chaerul Basri (Laboratory of Epidemiology, Division of Veterinary Public Health and Epidemiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia)
Etih Sudarnika (Laboratory of Epidemiology, Division of Veterinary Public Health and Epidemiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia)
Leovritta Masalun (Study Programs of Veterinary Medicine, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia)
Dinishaa Pasamurli (Study Programs of Veterinary Medicine, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, Indonesia)



Article Info

Publish Date
01 Oct 2026

Abstract

This study evaluated the effects of bacteriophage concentration and contact time on Enterobacteriaceae counts in chicken meat inoculated with Salmonella Typhimurium ATCC 14028. A cocktail bacteriophage preparation, VAM-S (SK-8 Biotech), was applied at four dilutions (1:1, 1:10, 1:100, and 1:1000), together with negative and positive controls, and the samples were stored under refrigeration for 0, 24, and 48 h. Because Enterobacteriaceae were used as the microbiological endpoint, the observed reductions should be interpreted as an indirect indicator of microbiological control rather than as direct enumeration of Salmonella alone. At 0 h, the inoculated groups started from broadly comparable baseline levels, ranging from approximately 2.68 to 4.01 log₁₀ CFU/g. Data from the inoculated groups were analyzed using two-way ANOVA, which showed significant main effects of treatment (p = 0.0066) and storage time (p = 8.24 × 10⁻⁵), with no significant interaction between treatment and storage time (p = 0.0834). At 48 h, the 1:10 dilution showed the largest numerical reduction relative to the positive control, with an estimated reduction of 0.906 log₁₀ based on the ratio of the mean CFU/g values. Visual observation did not reveal apparent changes in meat color, surface moisture, or texture following phage treatment. These findings suggest that VAM-S may contribute to the microbiological quality of chilled chicken meat, although the reduction observed under the present conditions was modest.

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Journal Info

Abbrev

JMV

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Veterinary

Description

urnal Medik Veteriner (JMV) publishes high quality and novelty papers focusing on Veterinary and Animal Science. The fields of study are anatomy, pathology, basic medicine, veterinary public health, microbiology, veterinary reproduction, parasitology, animal husbandry and animal welfare. Food ...