<![CDATA[Eastern little tuna (Euthynnus affinis) is a widely consumed marine fish in Indonesia and is known to serve as a paratenic host for zoonotic parasites. This study aimed to detect the presence of Anisakis sp. larvae in E. affinis obtained from two traditional fish markets in Mataram, West Nusa Tenggara, Indonesia: Traditional Kebon Roek Market and Bintaro Fish Market. A total of 50 fish samples were collected using simple random sampling. Enzymatic digestion method was applied to detect Anisakis sp. larvae from visceral organs and muscle tissue separately. Morphological identification was performed under stereo and compound microscopy at 10× magnification. Results showed that 5 of 50 samples (10%) were positive for Anisakis sp. larvae, with a mean intensity of 1 larva per infected fish. All positive larvae were recovered exclusively from visceral organs, while no larvae were detected in muscle tissue. Morphological examination confirmed the larvae as Anisakis sp. Type I, characterized by the presence of a boring tooth, elongated ventriculus, and mucron at the caudal end. These findings demonstrate that E. affinis traded in traditional markets of Mataram carries Anisakis sp. at a relatively low infestation level, yet poses a potential zoonotic risk. Proper post-harvest handling and adequate cooking are essential to prevent anisakiasis in consumers.]]>
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