Fungal skin infections remain a common health problem in Indonesia, primarily due to the humid tropical climate and poor environmental hygiene. Penicillium is one fungal genus that can be found on the skin, though it is not a primary dermatophyte. This study aims to detect the presence of Penicillium sp. molds and examine their macroscopic and microscopic morphological characteristics isolated from skin scrapings of agricultural worker. The research used a qualitative descriptive method, with skin scrapings cultured on Sabouraud Dextrose Agar (SDA). Macroscopic characterization included colony color, reverse color of the colony, diameter, margins, surface texture, and growth patterns. Microscopic analysis employed the Slide Culture method with lactophenol cotton blue (LPCB) staining. Results revealed green colonies with white margins, irregular colony growth, velvety surfaces, and septate hyphae, colony diameter after one week incubation 23.5mm. Conidia appeared round and arranged in chains. Observations were compared with fungal identification keys for genus confirmation. This study is expected to provide data on the distinct morphology of Penicillium sp. and improve diagnostic accuracy for non-dermatophyte fungal skin infections in laboratory settings.
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