AcTion: Aceh Nutrition Journal
Vol 11, No 3 (2026): September

Characterization of nutritional composition and bioactive compound of cowpea tempeh with moringa leaf powder as a functional food candidate for type 2 diabetes mellitus

Irma Sarita Rahmawati (Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya, Kabupaten Malang)
Rahmawati Indira Lahita (Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya, Kabupaten Malang)
Nindiya Muslima Febrianti (Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya, Kabupaten Malang)
Azizah Nurlaila (Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya, Kabupaten Malang)
Aulia Yafi’ Ramadhina (Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya, Kabupaten Malang)
Ginata Ayu Maulana (Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya, Kabupaten Malang)



Article Info

Publish Date
16 Sep 2026

Abstract

Diabetes mellitus (DM) is a chronic metabolic disorder that requires effective management, including dietary interventions. Functional foods, such as tempeh, enriched with bioactive ingredients, including cowpea and moringa leaf powder, may contribute to blood glucose regulation based on their fiber and antioxidant composition. This study aimed to evaluate the nutritional and bioactive properties of tempeh made from cowpea and moringa leaf powder and determine the optimal formulation for managing type 2 diabetes. A laboratory-based experimental design was conducted from September 2024 at the Diet and Culinary Laboratory, Universitas Brawijaya, using four formulations (T0, T1, T2, and T3) with varying proportions of cowpea and moringa leaf powders. The study sample consisted of cowpea and moringa leaf powders as raw materials for tempeh production. The analyses were limited to the proximate nutritional composition and selected bioactive compounds (vitamin C, antioxidant activity, and anthocyanin content). The results indicated that The best formulation was T2 (94% cowpea: 6% moringa leaf powder), which exhibited a moisture content of 58.93%, ash content of 1.50%, protein content of 14.90%, fat content of 0.39%, carbohydrate content of 24.27%, energy of 160.25 kcal, starch content of 8.86%, total sugar content of 12.73%, iron content of 15.3, vitamin C of 8.66, and antioxidant activity with an IC₅₀ value of 48.77. However, anthocyanin content was not detected in this study. Statistically significant differences were observed in the ash (p= 0.027), fat (p= 0.014), iron (p = 0.016), and antioxidant activity (p < 0.000) content. In conclusion, T2 shows promise as a functional food for type 2 diabetes management.

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Journal Info

Abbrev

an

Publisher

Subject

Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Description

AcTion: Aceh Nutrition Journal merupakan jurnal gizi dan kesehatan dengan E-ISSN 2548-5741 dan ISSN 2527-3310. Jurnal ini bertujuan untuk meningkatkan kemampuan dalam penyampaian hasil penelitian sebagai media yang dapat digunakan untuk meregistrasi, mendiseminasi, dan mengarsipkan karya peneliti ...