<![CDATA[Tissue culture is a plant propagation technique that can be used to produce orchid planting materials under controlled conditions. The process involves several stages, including explant preparation, aseptic handling, culture-medium preparation, shoot multiplication, rooting, and acclimatization. This study aimed to describe tissue culture practices for the in vitro propagation of Dendrobium sp. at the Sri Soedewi Orchid Garden in Jambi, Indonesia. A descriptive approach was employed based on observations and photographic documentation collected from 16 to 23 August 2023. The aspects described included culture-medium preparation, explant handling and sterilization, inoculation and culture transfer, shoot multiplication, rooting, and acclimatization. Murashige and Skoog (MS) medium was reported as the medium used in the observed culture activities. The findings are presented as descriptions of the documented procedures and technical practices. However, quantitative data on shoot number, shoot length, contamination rate, and acclimatization survival were unavailable; therefore, the effectiveness of the propagation technique could not be quantitatively evaluated. This study provides a descriptive account of orchid tissue culture practices and highlights the importance of systematic growth monitoring to support the evaluation and further development of propagation techniques in future research.]]>
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