Sintesis Wax Ester dari Asam Lemak Stearat secara Enzimatis Sebagai Bahan Baku Kosmetik

Roushandy Asri Fardani (Jurusan Analis Kesehatan, Politeknik Medica Farma Husada, Indonesia.)
Erin Ryantin G (Fakultas MIPA, Universitas Sebelas Maret, Indonesia.)

JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya
Vol. 3 No. 1 (2019)

The purpose of this study is to synthesize wax ester from fatty acid stearic with oleil alcohol, determine theoptimum conditions and to find out what percentage of wax ester is produced. Wax ester synthesis iscarried out enzymatically. Qualitative wax ester analysis using TLC and FT-IR, quantitatively by acid-basetitration and determination of optimum conditions using a variation of time, temperature, number ofenzymes. Based on the results of the TLC analysis obtained the Rf value of 0.14, FT-IR spectrum of stearicfatty acids, oleil alcohol and wax ester can be concluded that wax ester can be synthesized from fatty acidstearic with oleil alcohol enzymatically. The optimum conditions for wax ester synthesis from stearic fattyacid for 2 hours, at 40 ° C, with a lipase amount of 0.15 grams, in the ratio of stearic acid: oleil alcohol =1: 3 and with n-hexane as the best solvent. The percentage of conversion obtained is 94%.