Identifikasi Fitosterol dengan Kromatografi Gas – Spektrometer Massa pada Ekstrak Kloroform Biji Buah Atung (Parinarium Glaberimum Hassk) Asal Kabupaten Seram Bagian Barat, Maluku

Candra Yulius Tahya (Program Studi Pendidikan Kimia, Fakultas Ilmu Pendidikan, Universitas Pelita Harapan, Indonesia.)
Elen Tiwery (SMP Negeri 1 Kairatu Barat, Maluku, Indonesia)
Mega Gracelia Monaten (Fakultas Kedokteran, Univeritas Pattimura, Indonesia)
Tryani K. Junita Lumbantoruan (Fakultas Pertanian, Universitas Pattimura, Indonesia)

JC-T (Journal Cis-Trans) : Jurnal Kimia dan Terapannya
Vol. 4 No. 1 (2020)

One of the natural potentials that can be used as traditional medicine and natural preservatives is theseeds of Atung which can be found in Ambon, Seram and Lease Islands. The purpose of this study was toidentify bioactive phytosterols in the chloroform extract of Atung fruit seeds. Five main compounds have beenidentified in the chloroform extract of Atung fruit seeds (Parinarium glaberimum Hassk) which are linoleicacid (8.88%); stearic acid (8.23%); stigmasta-5,22-dien-3-ol,(3β,22E) or stigmasterol (10.37%); stigmast-5-en-3-ol,(3β) or β-sitosterol (19.59%); and stigmast-5-en-3-ol, (3β, 24S) or γ-sitosterol ( 33.74%) through gaschromatography-mass spectroscopy by analyzing the similarity of molecular weight and positive ionmass/charge fragments and based on the comparison of the GC-MS retention time of phytosterols. This studyis the first to report the presence of stigmasterol, β-sitosterol and γ-sitosterol in the chloroform extract ofAtung fruit seeds (Parinarium glaberimum Hassk). γ -Sitosterol, the epimer of β-sitosterol, has the greatestabundance in the chloroform extract of Atung fruit seeds.