<![CDATA[Dahu (Dracontomelon dao) belongs to the Anacardiaceae family which contains various secondarymetabolites that can be used as a source of natural antioxidants. The aim of this study was to extractsecondary metabolites from stems bark of D. dao, determine their antioxidant activity and analyze the contentof secondary metabolites using LC-MS/MS. The stem samples of D. dao were extracted in stages with varioustypes of solvents such as methanol ethyl acetate, and n-hexane. Each extract was analyzed for its antioxidantactivity using the 2,2-diphenyl-1 picrylhydrazyl (DPPH) method. The most active extracts werecharacterized by LC-MS/MS. The stem methanol extract of D. dao showed the best antioxidant activity withan IC50 value of 30.86 ppm, followed by ethyl acetate extract with an IC50 of 200.83 ppm and n-hexane extractwith an IC50 value of 348 ppm. Identification results with LC-MS/MS methanol extract containingtributylamine, medroxyprogesterone acetate, and flavanones.]]>
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