<![CDATA[Autism spectrum disorder (ASD) is associated with gut-brain axis (GBA) dysfunction, a bidirectional mechanism that plays an important role in maintaining gut and brain homeostasis. Gut microbiota disturbances may affect psychological, behavioral, and cognitive conditions in individuals with ASD. Modified cassava flour (MOCAF) and white millet seeds have potential as gluten-free food ingredients to support gut microbiota balance. This experimental study developed three GluMie formulations: F0 (100% wheat flour), F1 (60% white millet seeds and 40% MOCAF), and F2 (60% MOCAF and 40% white millet seeds). Sensory quality testing was conducted by three trained panelists, while the hedonic test involved 15 special-needs teachers as untrained panelists. Acceptability testing was conducted on 10 children with ASD by providing 30 g of F1 and F2. Gluten content and proximate composition were analyzed. The Friedman test showed no significant difference between F2 and F0 (p=0.059), whereas F1 differed significantly from F0 (p=0.003). Therefore, F2 was selected as the best formulation, containing 39.16% carbohydrates, 8.03% protein, 2.25% fat, 49.15% moisture, and 209.01 kcal of energy, and was confirmed to be gluten-free. The acceptability test showed 0% food waste. Thus, GluMie F2 has potential as a gluten-free food alternative for individuals with ASD.]]>
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