<![CDATA[Porang (Amorphophallus muelleri Blume) is a plant with high economic value whose conventional propagation is limited by the availability of planting material. An alternative to overcome this limitation is in vitro propagation. Unfortunately, in vitro propagation is often hampered by tissue browning caused by oxidation of phenolic compounds. This study aimed to determine the effects of polyvinylpyrrolidone (PVP) addition and incubation conditions on porang browning, shoot induction, and shoot propagation. The medium used was Murashige and Skoog (MS) containing 3 ppm BAP and 0.05 ppm NAA. A completely randomised design of two factors was used in this study. The first factor was PVP concentration of 0, 0.3, and 0.5 g/L, and light and dark conditions were the second factor. The explants were bubils, and there were five replicates. The dependent variables were 1) percentage of browning, 2) time of shoot emergence, and 3) number of shoots per explant. The results showed that incubation in dark conditions with PVP concentration of 0.3 g/L at the shoot induction stage showed the lowest browning percentage, which was 50%. Meanwhile, incubation under light conditions caused the explants to browning of 100% in all PVP treatments. Incubation under light conditions with a PVP concentration of 0.3 g/L was favourable response in accelerating shoot emergence, which was an average of 14 days after culture and produced the highest number of shoots compared to other treatments, averaging eight shoots per explant. To facilitate the large-scale production of superior porang planting material, this study offers a scientific basis for creating an effective and reproducible tissue culture methodology.]]>
Copyrights © 2026
