Agrovigor: Jurnal Agroekoteknologi
Vol 19, No 2 (2026): In Progress

Molecular characterization of a Bean common mosaic virus isolate inciting severe symptoms on yardlong bean in Gunungkidul Regency, Indonesia.

Rossa Wulandari (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Indonesia)
Ida Afifatun Nisa' (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Indonesia)
Aulia Diva Surya Putri (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Indonesia)
Alyah Shufa (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Indonesia)
Ummu Azhizah Nuraini (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Indonesia)
Septiana Septiana (State Polytechnic of Lampung, Jl. Soekarno Hatta No. 10, Kota Bandar Lampung, Lampung, 35141, Indonesia)
Efriandi Efriandi (South Sumatra Provincial Agriculture Office)
Adhyatma Noor Rizal (Center for Health and Human Nutrition, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada)
Adyatma Irawan Santosa (Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Indonesia)



Article Info

Publish Date
14 Sep 2026

Abstract

In 2023, an outbreak of viral disease on yardlong bean (Vigna unguiculata ssp. sesquipedalis) was reported in Tanjungsari District of Gunungkidul Regency, Indonesia. Symptomatic leaves showing mosaic, chlorosis, severe blistering, and leaf malformation were collected as a composite sample and then molecularly tested with RT-PCR. The sample was positive for potyvirus infection using U341/Poty1 universal primer pair, and BLAST analysis on the obtained nucleotide sequence further specified bean common mosaic virus (BCMV, Potyvirus phaseovulgaris) as the causal agent of the disease. Sequence of 691 bp of 3’-end region of BCMV-GKD-Ida isolate was registered in NCBI GenBank with accession no. PZ118702. The phylogenetic tree constructed using the Maximum Likelihood method with Tamura 3-parameter substitution model clustered BCMV-GKD-Ida with other BCMV isolates from 17 countries but in a distinct monophyletic branch. A paired identity analysis using the Sequence Demarcation Tool (SDT) software version 1.3 estimated that BCMV-GKD-Ida shares a fairly low nucleotide identity of 86.5%-92.5% with the compared BCMV isolates, further indicating considerable genetic variation. BCMV is one of most important plant pathogens that threaten the productivity legumes. This research provides additional molecular data and contributes a basis for early diagnosis and control strategies for the virus in yardlong bean.

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Journal Info

Abbrev

agrovigor

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Agrovigor: Jurnal Agroekoteknologi is a scientific paper in the field of science Agroecotechnology which include: plant science, soil science, plant breeding, pest and plant diseases. ...