<![CDATA[This exploratory in vitro study compared the rumen bacterial composition associated with two total mixed rations (TMR): a ration containing 10% Indigofera (Indigofera zollingeriana) pellets (P1) and a ration containing 10% Edamame (Glycine max) pellets (P2). Both rations contained 21-day fermented Pakchong grass silage, a commercial concentrate, and cassava peels. The feed samples were incubated with rumen fluid from one fistulated Bali cow at 39°C for 72 h. DNA was extracted, the V4 region of the 16S rRNA gene was sequenced, and the taxonomic relative abundance was summarized descriptively. Clostridia predominated in both treatments (45.34% in P1 and 50.62% in P2, respectively). P1 had higher abundance of Bacteroidia (16.95% vs. 9.23%), Negativicutes (10.85% vs. 6.46%), and Gammaproteobacteria (7.94% vs. 4.74%), whereas P2 had higher abundance of Coriobacteriia (16.37% vs. 2.36%). In the standalone phylum comparison, Bacillota predominated at 56.20% in P1 and 56.13% in P2; Bacteroidota and Pseudomonadota were higher in P1, whereas Actinomycetota was higher in P2. Because the study used a single rumen fluid donor and no inferential statistical test was reported, these findings describe treatment-associated patterns and do not establish statistical significance or improved fermentation efficiency.]]>
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