<![CDATA[Kopasanda leaves (Chromolaena odorata L.) contain flavonoids, tannins, saponins, and alkaloids, whichhave potential antibacterial activity. This study aimed to evaluate the antibacterial activity of n-hexane,ethyl acetate, and aqueous fractions of the ethanolic extract of kopasanda leaves against Staphylococcusaureus, as well as to determine the most active fraction, Minimum Inhibitory Concentration, and MinimumBactericidal Concentration. Extraction was performed using the maceration method with 70% ethanol,followed by fractionation using n-hexane, ethyl acetate, and water. Antibacterial activity was evaluatedusing the disk diffusion method at concentrations of 20, 10, and 5% to determine the most active fraction,followed by the dilution method at concentrations of 50; 25; 12.5; 6.25; 3.12; 1.56; 0.78; and 0.39% todetermine the Minimum Inhibitory Concentration and Minimum Bactericidal Concentration. Diffusiontest results were statistically analyzed using one-way analysis of variance to determine differences ininhibition zones among fractions. Thin-layer chromatography analysis showed that the extract and allfractions contained flavonoids, alkaloids, and tannins, except for the aqueous fraction, which only showedthe presence of saponins. The ethyl acetate fraction was the most active, with an inhibition zone diameterof 26.36 mm at a concentration of 20%. The Minimum Inhibitory Concentration could not be determineddue to the dark color of the fraction, while the Minimum Bactericidal Concentration of the ethyl acetatefraction against Staphylococcus aureus was 12.5%.]]>
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