This study evaluated fermented chicken manure and rice-washing water (air leri) as partial substitutes for rice bran in white oyster mushroom (Pleurotus ostreatus) growing media, aiming to reduce dependence on rice bran without lowering productivity. We used a completely randomized design with two factors: factor A (fermented chicken manure, 3% and 9%) and factor B (air leri, 10% and 20%), with four treatments and five replications. We observed and analysed mycelial colonization time and fresh fruiting-body weight at the first harvest using ANOVA followed by the least significant difference test. Both factors had a highly significant effect (P<0.01) on both variables, whereas their interaction was not significant. The fastest colonization (19 days) and the highest fresh weight (157.56 g) were obtained in treatment A2B2 (9% fermented chicken manure + 20% air leri), while the slowest and lowest were in A1B1 (29 days; 85.41 g). The combination of fermented chicken manure and air leri reduced rice-bran use by up to 75% while improving productivity, offering a low-cost substitution strategy that valorises livestock and household waste.
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