<![CDATA[Cyanthillium cinereum (L.) H. Rob. contains secondary metabolites with potential antibacterial activity. This study performed qualitative phytochemical screening of the leaf ethanol extract, formulated topical creams containing 0.75% (F1), 1.5% (F2), and 3% (F3) extract, and evaluated preliminary physical stability and antibacterial activity against Staphylococcus aureus ATCC 25923. Qualitative screening detected alkaloids, steroids, saponins, flavonoids, tannins, glycosides, and phenolic compounds. The creams were homogeneous; mean pH values ranged from 6.22 to 6.45 before cycling and from 6.00 to 6.05 after cycling. Antibacterial responses increased with concentration. F3 produced the greatest response among the creams (19.07 ± 0.45 mm, n = 3), while the blank base showed no inhibition; the corresponding unformulated 3% extract produced 19.23 ± 0.42 mm. Two-way ANOVA showed significant effects of preparation type (p < 0.001), concentration (p < 0.001), and their interaction (p = 0.003). The E3-F3 difference was not significant by Tukey HSD (p = 0.996), but this does not establish statistical equivalence. These findings indicate that an antibacterial response remains measurable after incorporation into a cream matrix and support further investigation of the 3% formulation.]]>
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