<![CDATA[The growing adoption of telemedicine highlights the importance of understanding factors influencing patient acceptance, particularly digital health literacy and trust. This study examined the mediating role of patient trust in the relationship between digital health literacy and telemedicine acceptance among MySiloam users at Siloam Hospitals Kebon Jeruk, Jakarta, Indonesia. A quantitative cross-sectional study involved 200 adult patients selected through purposive sampling. Data were collected using a 15-item Likert-scale questionnaire and analyzed through Pearson correlation, multiple regression, and PROCESS Macro Model 4 with 5,000 bootstrap samples. Results indicated that digital health literacy significantly predicted patient trust (β = 0.467, p < .001) and telemedicine acceptance (β = 0.533, p < .001). Patient trust also significantly predicted telemedicine acceptance (β = 0.461, p < .001). The indirect effect was statistically significant (B = 0.220, 95% CI [0.146, 0.303]), supporting partial mediation. These findings suggest that digital health literacy and patient trust jointly contribute to telemedicine acceptance. The study highlights the importance of strengthening digital health literacy alongside trust-building strategies emphasizing privacy, service reliability, and physician–patient communication to support telemedicine adoption in Indonesian healthcare settings.]]>
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