<![CDATA[Fasting blood glucose testing is a crucial laboratory procedure for diagnosing diabetes mellitus. Test result accuracy is influenced by the pre-analytical phase; specifically, delays in testing can lead to a decrease in glucose levels due to glycolysis. This study aimed to determine the difference in fasting blood glucose levels between immediate testing and testing delayed by three hours in patients with diabetes mellitus at Karisma Cimareme Hospital. An observational analytic design with a comparative approach was employed, involving 30 patients with diabetes mellitus. Fasting blood glucose levels were measured using the Glucose Oxidase Peroxidase Aminoantipyrine (GOD-PAP) method. Data were analyzed using the Shapiro-Wilk normality test followed by a paired-sample t-test. The results showed that the mean fasting blood glucose level was 180.27 mg/dL for immediate testing and 165.53 mg/dL for testing delayed by three hours, representing a mean decrease of 14.73 mg/dL. The paired-sample t-test yielded a p-value of <0.001 (p < 0.05), indicating a significant difference between the two testing conditions. The study demonstrates that a three-hour delay in testing contributes to a reduction in fasting blood glucose levels; therefore, testing should be performed promptly.]]>
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