Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Vol 8, No 1 (2013): May 2013

MOLECULAR IDENTIFICATION OF SPONGES OBTAINED FROM SERIBU ISLANDS NATIONAL PARK AND THEIR ASSOCIATED BACTERIA

Patantis, Gintung (Unknown)
Rahmadara, Gemilang (Unknown)
Elfidasari, Dewi (Unknown)
Chasanah, Ekowati (Unknown)



Article Info

Publish Date
25 May 2013

Abstract

Sponges are simple multicellular animals that produced many pharmaceutical secondary metabolites. Some sponge-associated bacteria are proven to produce the same metabolites as their host, giving an opportunity to mass produce the potential metabolites. The aim of this research was to analyze the diversity of sponge-associated bacteria and to identify  the host sponge. Samples were collected from Seribu Islands National Park. Partial identification of sponges were conducted by molecular technique with the mitochondrial cytochrome oxidase subunit 1 (CO1) as the target area. The diversity of sponge-associated bacteria was determined by Terminal Restriction Fragment Length Polymorphism (T-RFLP) method. Result showed that sponges PS-17-12 has similarity with Petrosia sp., while PS-26-12 and PS-38-12 has similarity with Xestospongia muta. From the 3 sponge samples, 85 species of bacteria was obtained which can be classified into 9 phylums and 1 uncultured bacteria/environment sample. Some of  the sponge-associated bacteria identified were known as a potential producer of metabolites with antibiotic activity.

Copyrights © 2013






Journal Info

Abbrev

squalen

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Description

Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be ...