Jurnal Penelitian Kelapa Sawit
Vol 24 No 2 (2016): Jurnal Penelitian Kelapa Sawit

OPTIMASI PROTOKOL EKSTRAKSI DNA Elaeidobius kamerunicus

Sri Wening (Indonesian Oil Palm Research Institute (IOPRI))
Agus Eko Prasetyo (Indonesian Oil Palm Research Institute (IOPRI))
Tjut Ahmad Perdana Rozziansha (Indonesian Oil Palm Research Institute (IOPRI))
Agus Susanto (Indonesian Oil Palm Research Institute (IOPRI))



Article Info

Publish Date
01 Aug 2016

Abstract

African pollination weevil (Elaeidobius kamerunicus Faust) has an important role in the productivity of Indonesian oil palm plantation. Up to now, there has not been a comprehensive biological study of the species at molecular level. The basic knowledge is very useful for exploitation of the weevil for effective oil palm fruit set development. This research aimed to obtain DNA extraction protocol of E. kamerunicus for DNA fingerprinting of the species. Results showed that using a DNA extraction kit,material disruption by using micro pestle resulted the highest quantity of DNA, while there were no significant differences of resulted DNA quantity among treatments using tissue lyser for material disruption. DNA extracted by using micro pestle or tissue lyser for material disruption is adequate for DNA fingerprinting using AFLP (Amplified Fragment Length Polymorphism) and sequencing techniques.

Copyrights © 2016






Journal Info

Abbrev

jpks

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Indonesian Journal of Oil Palm Research Volume 26 Number 2 Year 2018 is published by presenting articles: Utilization of candlenut shell charcoal (Aleurites moluccana (L.) Willd.) as adsorben on refinery of Crude Palm Oil (CPO); Application of an Artificial Neural Network (ANN) model for predicting ...