Indonesian Aquaculture Journal
Vol 9, No 2 (2014): (December 2014)

MICROBIAL COMMUNITY OF BLACK BAND DISEASE ON INFECTION, HEALTHY, AND DEAD PART OF SCLERACTINIAN Montipora sp. COLONY AT SERIBU ISLANDS, INDONESIA

Ofri Johan (Research and Development Institute for Ornamental Fish Culture, Jakarta)
Dietriech Geoffrey Bengen (Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, Bogor Agricultural University)
Neviaty Putri Zamani (Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, Bogor Agricultural University)
Suharsono Suharsono (Research Center for Oceanography, The Indonesian Institute of Science, Jakarta)
David Smith (School of Biology, Newcastle University, United Kingdom)
Angela Mariana Lusiastuti (Research and Development Institute for Freshwater Aquaculture, Bogor)
Michael Sweet (Biological Sciences Research Group, University of Derby, United Kingdom)



Article Info

Publish Date
31 Dec 2014

Abstract

It is crucial to understand the microbial community associated with the host when attempting to discern the pathogen responsible for disease outbreaks in scleractinian corals. This study determines changes in the bacterial community associated with Montipora sp. in response to black band disease in Indonesian waters. Healthy, diseased, and dead Montipora sp. (n = 3 for each sample type per location) were collected from three different locations (Pari Island, Pramuka Island, and Peteloran Island). DGGE (Denaturing Gradient Gel Electrophoresis) was carried out to identify the bacterial community associated with each sample type and histological analysis was conducted to identify pathogens associated with specific tissues. Various Desulfovibrio species were found as novelty to be associated with infection samples, including Desulfovibrio desulfuricans, Desulfovibrio magneticus, and Desulfovibrio gigas, Bacillus benzoevorans, Bacillus farraginis in genus which previously associated with pathogenicity in corals. Various bacterial species associated with uninfected corals were lost in diseased and dead samples. Unlike healthy samples, coral tissues such as the epidermis, endodermis, zooxanthellae were not present on dead samples under histological observation. Liberated zooxanthellae and cyanobacteria were found in black band diseased Montipora sp. samples.

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Journal Info

Abbrev

iaj

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine ...