Indonesian Journal of Agricultural Science
Vol 19, No 2 (2018): December 2018

ANALYSIS OF GENETIC DIVERSITY IN TWELVE CULTIVARS OF PEA BASED ON MORPHOLOGICAL AND SIMPLE SEQUENCE REPEAT MARKERS

Brijesh Kumar Singh (Department of Agricultural Biotechnology Bidhan Chandra Krishi Viswavidyalaya Mohanpur-741252, Nadia(W.B))
Monoj Sutradhar (Department of Agricultural Biotechnology Bidhan Chandra Krishi Viswavidyalaya)
Amit Kumar Singh (Central Agriculture University, College of Horticulture and Forestry)
Ajay Kumar Singh (Department of Genetics and Plant Breeding, Candra Shekar Azad University of Agriculture and Technology)
Rajendra Prakash Vyas (Department of Genetics and Plant Breeding, Candra Shekar Azad University of Agriculture and Technology)



Article Info

Publish Date
09 Dec 2018

Abstract

Pea(Pisum sativum L.)is the second most important legume crop worldwide after chickpea (Cicer arietinum L.) and valuable resources for their genetic improvement. This study aimed to analyze genetic diversity of pea cultivars through morphological and molecular markers. The present investigation was carried out with 12 pea cultivars using 28 simple sequence repeat markers. A total of 60 polymorphic bands with an average of 2.31 bands per primer were obtained. The polymorphic information content, diversity index and resolving power were ranged from 0.50 to 0.33, 0.61 to 0.86 and 0.44 to 1.0 with an average of 0.46, 0.73 and 0.76, respectively. The 12 pea cultivars were grouped into 3 clusters obtained from cluster analysis with a Jaccardd’s similarity coefficient range of 0.47-0.78, indicating the sufficient genetic divergence among these cultivars of pea. The principal component analysis showed that first three principal components explained 86.97% of the total variation, suggesting the contribution of quantitative traits in genetic variability. The contribution of 32.59% for number of seeds per plant, stem circumference, number of pods per plant and number of seeds per pod in the PC1 leads to the conclusion that these traits contribute more to the total variation observed in the 12 pea cultivars and would make a good parental stock material. Overall, this SSR analysis complements morphological characters of initial selection of these pea germplasms for future breeding program.

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Journal Info

Abbrev

IJAS

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

The journal publishes primary research articles from any source if they make a significant original contribution to the experimental or theoretical understanding of some aspect of agricultural science in Indonesia. The definition of agricultural science is kept as wide as possible to allow the ...