Media Penelitian dan Pengembangan Kesehatan
Vol 23, No 2 Jun (2013)

EFEKTIVITAS Bacillus thuringiensis H-14 STRAIN LOKAL DALAM BUAH KELAPA TERHADAP LARVA Anopheles sp dan Culex sp di KAMPUNG LAUT KABUPATEN CILACAP

P, Blondine Ch. ( Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit Salatiga, Balitbangkes, Kemenkes RI, Jl. Hasanudin No. 123, Salatiga, JawaTengah, Indonesia)
Widyastuti, Umi ( Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit Salatiga, Balitbangkes, Kemenkes RI, Jl. Hasanudin No. 123, Salatiga, JawaTengah, Indonesia)



Article Info

Publish Date
30 Jul 2013

Abstract

Abstrak Bacillus thuringiensis serotipe H-14 strain lokal adalah bakteri patogen bersifat target spesifiknya larva nyamuk, aman bagi mamalia dan lingkungan. Penelitian bertujuan menentukan efektivitas B. thuringiensis H-14 strain lokal yang dikembangbiakkan dalam buah kelapa untuk pengendalian larva Anopheles sp dan Culex sp. Rancangan eksperimental semu, terdiri dari kelompok perlakuan dan kontrol. Bacillus thuringiensis H-14 strain lokal dikembangbiakan dalam10 buah kelapa umur 6–8 bulan, dengan berat kira-kira 1 kg, telah berisi air kelapa sekitar 400-500 ml/buah kelapa yang diperoleh dari Desa Klaces, Kampung Laut, Kabupaten Cilacap. Diinkubasi selama 14 hari pada temperatur kamar dan ditebarkan di 6 kolam yang menjadi habitat perkembangbiakan larva nyamuk dengan luas berkisar 3–100 m2.Hasil yang diperoleh menunjukkan efektivitas B. thuringiensis H-14 strain lokal terhadap larva Anopheles sp dan Culex sp selama 1 hari sesudah penebaran kematian larva berturut-turut sebesar 80–100% dan 79,31–100%. Sedangkan pada hari ke-14 sebesar 69,30–76,71% dan 67,69–86,04%. Buah kelapa dapat digunakan sebagai media lokal alternatif untuk pengembangbiakan B. thuringiensis H-14 strain lokal Kata kunci: B. thuringiensis H-14,  strain  lokal, buah kelapa, pengendalian larva Abstract Bacillus thuringiensis serotype H-14 local strain is pathogenic bacteria which specific  target to mosquito larvae. It is safe for mammals and enviroment. The aims of this study was to determine the effectivity of B. thuringiensis H-14 local strain which culturing in thecoconut wates against Anopheles sp and Culex sp mosquito larvae. This research is quasi experiment which consist of treated  and control groups. Bacillus thuringiensis H-14 local strain was cultured in 10 coconuts with 6–8 months age with weight around 1 kg that contained were approximately 400-500 ml/coconut were taken from Klaces village, Kampung Laut. After that the coconuts incubated for 14 days in the room temperatur and applied to 6 ponds breeding habitat larvae with the width of samples  from  3–100 m2 . The results showed the effectivity of B. thuringiensis H-14 local strain against Anopheles sp and Culex sp mosquito larvae until 1 day after applied the larvae mortalities were 80–100% dan 79,31–100% respectively. Fourteen days after applied were 69,30–76,71% and 67,69–86,04%. Coconut water can be used as  alternative local media to culture B. thuringiensis local strain. Key words : B. thuringiensis H-14,  local strain, coconut, larvae control

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Journal Info

Abbrev

MPK

Publisher

Subject

Decision Sciences, Operations Research & Management Public Health

Description

Media Health Research and Development ( Media of Health Research and Development ) is one of the journals published by the Agency for Health Research and Development ( National Institute of Health Research and Development ) , Ministry of Health of the Republic of Indonesia. This journal article is a ...