Indonesia Chimica Acta
Volume 3 No 1 - June 2010

Optimasi Produksi Enzim Lipase Dari Isolat Aspergillus oryzae

Seniwati Dali (Departemen of Chemistry, Hasanuddin University)
Rauf Patong (Departemen of Chemistry, Hasanuddin University)
Muhammad Noor Djalaluddin (Departemen of Chemistry, Hasanuddin University)
Pirman AP (Department of Chemical Engineering, Ujung Pandang State of Polytechic)



Article Info

Publish Date
01 Jun 2010

Abstract

Production optimization of lipase enzyme from Aspergillus oryzae isolate on moldy copra has been conducted. Enzyme isolation is carried out after the fungi is activated and cultured on media containing peptone and olive oil at 370C in temperature and 7,0 in pH during 8 days. Production optimization of lipase enzyme is conducted by varying composition of production media, namely: peptone concentration and olive oil and optimal stirring speed. The study results showed that activity of the highest lipase enzyme is acquired on pepton concentration of 1 %, olive oil concentration of 3 % and the stirring speed is 150 rpm. Of concentration optimization of production media and stirring speed is acquired that there is an increasing activity of the lipase enzyme from 13.222 U/mL to 18888U/mL.

Copyrights © 2010






Journal Info

Abbrev

ica

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering

Description

Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) is a peer-reviewed research journal that is devoted to the dissemination of new and original knowledge in all branches of chemistry. The result of research and development in the fields of chemistry in both experimental and theory/ computation, ...