Jerami
Vol 1 No 1 (2018): JIJCS

Micropropagationof Male and Female Trees of Andaleh (MorusmacrouraMiq.) through In-vitro Culture using Several Compositionsof Basal Medium

Aswaldi Anwar (Andalas University)



Article Info

Publish Date
30 Aug 2018

Abstract

Andalehis the local name of MorusmacrouraMiq. in West Sumatra, Indonesia. Nowadays, this dioeciously speciesis in endangered situation. The aim of the research is to find out the appropriate combination of plant growth regulator to induce shoot multiplication of explants from male and female trees of andaleh. The plantlets from this research will be used in the next future to conserve this endangered species in vitro and in vivo, especially in preparing parental material in breeding program. Young buds from male and female trees were used as an explants in basal medium Murashige and Skoog supplemented with BAP (0.5, 1.0, 1.5 and 2.0 mg.L-1) in combination with NAA (1.0 mg.L-1 for each). The frequency of bud break was 50 % in MS medium supplemented with BAP (0.5 mg.L-1) and NAA 1.0 mg.L-1 for both source of explants (female and female trees of andaleh) after 3 weeks of culture. Generally, the number of shoot induction was very low. On the other hand, the rate of callus formation was high (100%) in highest BAP concentration (2.0 mg.L-1).

Copyrights © 2018






Journal Info

Abbrev

Jerami-JIJCS

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

JERAMI : Indonesian Journal of Crop Science is an open access and international peer-reviewed scientific journal publishing research articles, reviews and short communications in the field of crop sciences. Scope coverage of this journal includes: agronomy, crop physiology, seed science, ...