Jerami
Vol 1 No 2 (2019): JIJCS

The Application Of PCR RAPD Technique In Determining Female Sex Of Kapulasan (Nepheleum Lapaceum Mutabile)

Edi Wirman (Universitas Taman Siswa-Padang Sumatera Barat)



Article Info

Publish Date
28 Feb 2019

Abstract

Kapulasan is one of horticultural plants derived from tropical region that has significant economic value. One of the obstacles in cultivating the plant is that kapulasan has flowering type of androdioceus, i.e. the male and the female flowers are separated. Female flowers produce hermaphroditic flowers that can produce fruits while the male ones will not produce fruits. In the cultivation of the plant, it is very difficult to determine the female flowers if they are propagated by seeds. It takes up to 7 years to determine the nature of the flowering. One of the important steps that can be done is through a system of early detection using molecular marks. RAPD technique as a simple technique can be used to distinguish the characteristics. The results of the research show that of the 250 primers used, the OPM-09 primer produces female sex bands related to the controller of the female kapulasan plants of which the ribbon produced is 512 bp in size.

Copyrights © 2019






Journal Info

Abbrev

Jerami-JIJCS

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

JERAMI : Indonesian Journal of Crop Science is an open access and international peer-reviewed scientific journal publishing research articles, reviews and short communications in the field of crop sciences. Scope coverage of this journal includes: agronomy, crop physiology, seed science, ...