UCP3 gene polymorphism and insulin resistance in obese female adolescents

Dian Eurike (Department of Histology and Cell Biology, Gadjah Mada University Medical School, Yogyakarta)
Harry Freitag Luglio Muhammad (Department of Health Nutrition, Gadjah Mada University Medical School, Yogyakarta)
Rina Susilowati (Department of Histology and Cell Biology, Gadjah Mada University Medical School, Yogyakarta)
Madarina Julia (Department of Child Health, Gadjah Mada University Medical School, Yogyakarta)

Paediatrica Indonesiana
Vol 52 No 3 (2012): May 2012

Background A previous study on obese female adolescents inYogyakarta done in the year 2007 suggests that genetic factorsmight influence insulin resistance. One genetic factor that hasbeen associated \\lith insulin resistance in other populations isthe 􀁐55C;T polymorphism in the uncoupling protein 3 (UCP3)gene.Objective To investigate an association between the 􀁐55C;Tpolymorphism in the UCP3 gene and insulin resistance in obesefemale adolescents in Yogyakarta.Methods A total of79 obese female adolescents were enrolled inthis cross􀁐sectional study. Genotyping of the 􀁐 5 SC/T polymorphismin the UCP3 gene was performed by polymerase chain reaction􀁐restriction fragment length polymorphism (PCR􀁐RFLP).Results The TiT homozygous individuals had a higher risk ofinsulin resistance (OR 2.3; 95% CI 0.4 to 14.1), as well as higherfasting insulin concentration and homeostatic model assessmentfor insulin resistance (HOMA􀁐IR) compared to individuals v.ithother genotypes. The T allele carriers also had a higher risk ofinsulin resistance (OR 1.3; 95% CI 0.7 to 2.5), as well as higherfasting insulin concentration and HOMA􀁐 IR compared to Callele carriers. However, none of these results were statisticallysignificant (P > 0.05).Conclusion The TiT genotype and T allele of the UCP3 gene􀁐55CiT polymorphism was not significantly associated increasedrisk of insulin resistance in obese female adolescents in Yogyakarta.[Paediatr lndanes. 2012;52:152-6].