Jurnal Entomologi Indonesia
Vol 11 No 1 (2014): April

Asosiasi antara marka SSR dengan ketahanan terhadap wereng batang coklat pada varietas dan calon galur harapan padi

Chaerani Chaerani (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian)
Dwinita W. Utami (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian)
Nurul Hidayatun (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian)
Buang Abdullah (Balai Penelitian Padi)
Bambang Suprihatno (Balai Penelitian Padi)



Article Info

Publish Date
22 Sep 2015

Abstract

Development of resistant rice varieties to brown planthopper (BPH, Nilaparvata lugens Stål) must be countinuously conducted to overcome the spatial and temporal genetic change in BPH into more virulent ones which can adapt to resistance rice. The availability of linked molecular markers with BPH resistant trait can speed up selection of hybridization progenies. Close-linked markers with trait of interest can be identified using association studies between markers and phenotypic data of unrelated individuals without the need to generate hybridization progenies as an alternative method to linkage studies which use mapping population. The objective of this study was to analyze the association of rice simple sequence repeat (SSR) markers with resistance to BPH. Forty-four rice varieties and promising lines with known resistance degree to BPH biotype 3 were assessed using 30 rice microsatellite markers previously mapped in the rice chromosomal regions with effects on resistance to BPH. Association test between SSR markers and BPH resistance scores revealed eight markers (RM17, RM38, RM125, RM144, RM250, RM287, RM328, dan RM536) which were significantly associated with BPH resistance (R2=0,18 to 0,89; P<0,05). Twenty-one varieties and 11 promising lines were detected to contain SSR alleles that were associated with BPH resistance. Marker RM17 was detected in 15 varieties and 11 promising lines which were scored as resistant and moderately resistant to BPH and therefore it is potentially the most useful as early diagnostic marker to detect BPH resistance alleles. Nevertheless, to obtain SSRs that can be used as reliable selection markers for BPH resistance, marker analysis in segregating populations for the eight markers is still needed as well as association test of more SSR markers widely distributed in rice chromosomes.

Copyrights © 2014






Journal Info

Abbrev

jei

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

Jurnal Entomologi Indonesia (JEI) publishes original research papers and short communications that covers the basic and applied aspect of insects and mites or other arthropods in agriculture, forestry, human and animal health, and natural resources and environmental management. The journal is ...