Coptotermes curvignathus Holmgren is the most destructive subterranean termites which caused economic loss in some big city in Indonesia. Soldier of C. curvignathus Holmgren have a defensive secretions with white colour and sticky. These soldier defensive secretions are used to overcome their enemy. In this research, soldier defensive secretions (SDS) from C. curvignathus Holmgren were isolated and extracted in polar and non-polar solution and their antifungal activity was studied. The solution used for extraction was ethyl acetate, ethanol, aquadest and n-hexane. The fungal used to determine antifungal activity was Schizophyllum commune. The result showed that extract from ethyl acetate shown the highest inhibition zone meanwhile other extract have no activity. The compound then purified by GCMS (Gas Chromatography Mass Spectra) and found that aldehyde compound Pentadecanal and Tetradecanal were the bioactive compound. This was the first research about possibilities of soldier defensive secretions from C. curvignathus Holmgren as antifungal against Schizophyllum commune
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