Floribunda
Vol. 4 No. 8 (2014)

PHYLOGENETIC RELATIONSHIPS OF INDONESIAN BANANA CULTIVARS INFFERED FROM TRNL-F INTERGENIC SPACER OF CHLOROPLAST DNA

Amin Retnoningsih (Department of Biology, Faculty of Mathematics and Sciences, Semarang State University)
Rita Megia (Department of Biology, Faculty of Mathematics and Sciences, Bogor Agricultural University)
Alex Hartana (Department of Biology, Faculty of Mathematics and Sciences, Bogor Agricultural University)



Article Info

Publish Date
28 Feb 2016

Abstract

A phylogenetic relationship of Indonesian banana cultivars was conducted using trnL-F intergenic spacer of chloroplast DNA sequences. The ingroup was 39 banana accessions from various genomic groups in Indonesia consisting of 9 AA/AAA; 11 AAA; 2 BB; 13 AAB; and 4 ABB cultivars and 12 sequences of different taxa of Musa downloaded from GenBank consisting of 4 AA wild; 1 AA; 3 AAB; and 4 ABB cultivars. Two accessions from section Rhodoclamys and Australimusa were used as the outgroup. The 39 accessions of the ingroup and the 2 accessions of the outgroup were analyzed using primers of the trnL-F intergenic spacer. The results showed that sequence of the trnL-F region can clearly distinguish 40 banana accessions having the A-type chloroplast (AA wild; AA; AA/AAA; AAA; AAB; and ABB) from those 11 accessions having the B-type chloroplast (BB, AAB, and ABB). The A-type chloroplast accessions are most probably derived from female Musa acuminata, while the B-type chloroplast accessions may be derived from female M. balbisiana. However, the specific M. acuminata ancestor of each cultivar remains unidentified.

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Journal Info

Abbrev

Floribunda

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

Floribunda is published both in Bahasa Indonesia and English, covers wide range of plant diversity, taxonomy and systematics of Malesian flora particulary distributed in ...