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Jurnal Teknologi Laboratorium
Published by Poltekkes Kemenkes Yogyakarta
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Education,
Chemistry Other
Arjuna Subject : -
Articles 6 Documents
 1 
Search results for , issue "Vol 8 No 1 (2019): 2019 (1)" : 6 Documents clear
The Physiochemical properties, sensory evaluation and shelf life of corn flour supplemented with Acheta gossypii (cricket) flour Ehoche, Elijah Edache; Oluwafunmi, Akanya; Oluwafunmilola, Adefolalu Folasade
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (453.893 KB) | DOI: 10.29238/teknolabjournal.v8i1.150

Abstract

Most consumed diets are made from corn and are deficient in proteins. Corn flour was made from fermented and dried yellow corn and supplemented with 5 and 10 % proteins of crickets. The diets were analyzed for the following physiochemical properties: swelling index, wettability, bulk density, solubility, and water holding capacity as well as sensory characteristics in a 7 point hedonic scale and the maintaining quality of each diet. Standard methods for physiochemical properties were used, and the microbial assay was also to analyze the diets at refrigerated and non-refrigerated conditions. The swelling index(1.83-2.00 g), gelatinization temperature ( 66.3 -72.3°C), wettability (22.0-120 seconds), and bulk density (0.59 - 0.67 mg/mL), significantly increased but solubility (14.1 - 3.00%), and oil-water holding capacity ( 90-65 %) reduced significantly in the cricket supplemented diets over the corn flour ( p< 0.05). The sensory characteristics from both the adult and children scored above average on the 7 points hedonic scale, with a major preference for the 5 % cricket supplemented the diet. It is noteworthy that in the 4 weeks of shelf life experiment, all the diets were found to be devoid of the growth of coliforms, yeast and the only occurrence of the mold is below the harmful threshold except with the 10 % cricket supplemented non-refrigerated diet with the growth of B. subtilis on the 3rd week of the experiment. However, the growth of this bacterium is below a harmful threshold. Proper handling and processing are safest before consumption. Supplementations of corn flour with cricket have been found to improve the nutritional quality of corn and may, therefore, be able to solve the problem of malnutrition.
Purification and characterization of recombinant Streptokinase expressed in E.coli from Streptococcus equisimilis with N-terminal methionine Vadla, Pavan Kumar; Tummuru, Murali; Kumar, Dinesh
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.321 KB) | DOI: 10.29238/teknolabjournal.v8i1.153

Abstract

Streptokinase is a extracellular enzyme which is extracted from strains of beta Hemolytic streptococcus. The enzyme is a non-protease plasminogen activator that activates plasminogen to plasmin and degrades fibrin clot through its specific lysine binding site which is used in thrombolytic therapy. Purification of streptokinase produced from S.equisimilis in E.coli with N-terminal methionine was carried out in 3 Chromatography purification steps, 1) CM-Sepharose-FF at pH 4.2 followed by concentration and dialysis over night with Tris-HCl pH 8.0. Partially purified dialyzed enzyme sample was loaded on to 2) DEAE-Sepharose-FF column. The Purified fractions of DEAE column were pooled and applied on to Sephadex G-100 column. Enzyme purity was confirmed by SDS-PAGE and RP-HPLC.Its biological activity is determined by specific streptokinase assay and characterised the enzyme by Peptide mapping, MALDI-TOF, Isoelectric-focusing and RP-HPLC. The isoelectric point (pI) of streptokinase is around 4.98.The results of characterization shows that it contains two forms (Isomers) of streptokinase expressed in E. coli which was analyzed by RP-HPLC and chromogenic assay. The variation is formed by isomer-1 in which 85% of Streptokinase expressed without methionine (85000IU/mg) and Isomer-2 in which 15% of streptokinase expressed with methionine (nil activity) in E. coli. This phenomenon shows that the presence and absence of methionine in isomers of streptokinase varying the catalytic activity of the enzyme.
The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O Turista, Dora Dayu Rahma; Puspitasari, Eka
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.651 KB) | DOI: 10.29238/teknolabjournal.v8i1.155

Abstract

BAP media is a medium used to distinguish pathogenic bacteria based on their hemolytic power on red blood cells. Staphyllococcus aureus is a bacterium that is able to emolate red blood cells with 3 types of hemolysis, namely α, β, γ, and δ. Usually BAP media is made by adding 5-10% sheep blood. Making BAP media using sheep blood has become a problem for several laboratories today, including health education laboratories. This is because the health education laboratory does not yet have a sheep farm, so it has not been able to procure sheep blood. The use of human blood as a substitute for sheep blood in making BAP media may be a solution, but it is not yet known whether there are differences in the growth and hemolysis of S. aureus bacteria on BAP media in sheep's blood and human blood. This research is an experimental study with a completely randomized design (CRD) of 3 replications which aims to determine whether there are differences in growth and hemolysis of bacteria S. aureus in BAP media of sheep blood and human blood groups A, B, AB, and O. The results showed that S. aureus bacteria could grow and show hemolysis in BAP media in sheep blood and human blood in groups A, B, AB, and O. The results of subsequent studies analyzed ANOVA using the software spss for windows with a significant level of 0.05. From the results of research and data analysis it can be concluded that S. aureus bacteria can grow and show hemolysis in BAP media of sheep blood and human blood groups A, B, AB and O, but there are significant differences in the number of S. aureus bacteria colonies grown in BAP media of sheep's blood and human blood groups A, B, AB and O.
Erythrocyte sedimentation rate in diabetic and non-diabetic patients of cardiovascular disease Kiyani, Mubin Mustafa; Jahan, Saira; Kiyani, Sania Khawar; Rehman, Hamza; Khan, Lal Gul; Iqbal, Umar
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (189.077 KB) | DOI: 10.29238/teknolabjournal.v8i1.159

Abstract

The pervasiveness of global endemic of type II diabetes, driven by population development, increasing obesity, urbanization, and other additional unidentified factors may be crumpled in the next 20yrs. The purpose of this study was to determine the correlation of ESR in diabetic and non-diabetic patients of cardiovascular diseases. It is a co-relational study conducted at KRL hospital Islamabad from February 2018 to September 2018. The study population compromised a total of 180 cases aged 45 to 50 years were taken, in which 60 cases of CVD with DM, 60 cases of CVD without DM and 60 normal healthy population were taken. All patients and controls gave written informed consent. All subjects went through a general physical examination, and a questionnaire was used to collect the records about demographics and past medical history and existing use of medications. In the presented study, Pearson's Correlation between ESR of healthy and ESR of CVD without DM shows a weak correlation between these two variables i.e., 0.127 with a p-value of 0.503. We also establish that there is a direct intermediate relationship between ESR of healthy and ESR of CVD with DM. High level of ESR in CVD patients might indicate the prognosis of DM. To confirm this further researches and studies must be conducted in this area
The concentration of NaCl soaking to decreasing cyanide levels in Gadung (Dioscorea hispida Dennst) Kresnadipayana, Dian; Waty, Helmy Indra
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (239.726 KB) | DOI: 10.29238/teknolabjournal.v8i1.156

Abstract

Cyanide is one component contained in gadung tubers, and therefore, consuming gadung that contains more than 1 ppm of cyanide can cause nausea and intoxication. This research aims to study the effect of concentration variation of NaCl soaking to the decrease of cyanide levels in gadung. This research is an experimental study with three treatment. Testing is only done once. Samples of gadung were soaked by NaCl (0% 1% 3% 5%) for 3 days. Determination of cyanide content in gadung used the UV-Vis spectrophotometry method by adding Ninhydrin 0.1% in Na2CO3 2% and NaOH 0.1 % in the sample filtrate. Result of this research had shown that cyanide content in gadung before NaCl soaking was 4.42 ppm and after NaCl soaking (0% 1% 3% 5%) were 3.52; 2.53; 0.43 and 0.01 ppm, respectively.
RETN rs3745368 polymorphism and resistin level in Javanese ethnic Indonesian obese: a case control study Utami, Rizki Fajar; Hastuti, Pramudji; Sadewa, Ahmad Hamim
Jurnal Teknologi Laboratorium Vol 8 No 1 (2019): 2019 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.825 KB) | DOI: 10.29238/teknolabjournal.v8i1.164

Abstract

Obesity has become a global public health problem. It occurs due to a positive energy balance leading to adipose tissue expansion. White adipose tissue was an endocrine organ which secreted resistin. Resistin also produced by immune cells due to low chronic level inflammation might cause higher resistin level in obese people. Polymorphism +62G>A RETN gene was reported has a relationship with low resistin level and A allele as a protective allele. This study aimed to determine genotype and allele frequency distribution concerning resistin level. Another objective aimed to know the correlation between resistin level with body mass index. The design of the research was a case-control study with 122 people (18-40 y.o.), divided equally in the case group (BMI ≥ 27 kg/m2) and control group (BMI 18.5-24.9 kg/m2) without diabetes mellitus. Blood was taken after fasting a minimal 8 hours. Plasma was used to measure the resistin level. DNA genotyping was analyzed using PCR-RFLP. Genotyping result showed three genotypes of RETN gene +62G>A polymorphism (GG, GA, AA). There was no significant difference in genotype and allele frequency distribution related to obesity status (p=0.680; p=1) and resistin level (p=0.537) between case and control group. There was no significant difference in resistin level between case and control group (p=0.770). Resistin level was correlated with BMI in obese group (p= 0.05; r= -0.25). The present study concludes that there is no significant difference in genotype and allele frequency distribution related to obesity status and resistin level. Resistin level has a negative correlation with BMI.

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