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Medical Laboratory Technology Journal

JAK
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Published by Poltekkes Kemenkes Banjarmasin

ISSN : - EISSN : 24610879 DOI : https://doi.org/10.31964/mltj

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health

The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 8 Documents

Search results for , issue "Vol. 5 No. 1 (2019): June" : 8 Documents clear

Association of the TG/HDL-C and Monocyte Count/HDL-C Ratios with Coronary Artery Disease in Non-ST Segment Elevation Myocardial Infarction Uğur Küçük; Recep Kurt
Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Monocyte Count/HDL ratio (MHR) is a simple and inexpensive parameter used as a risk predictor in patients diagnosed with myocardial infarction (MI). This study aims to investigate the association between Triglyceride/HDL-C (TG/HDL-C) ratio and coronary artery disease in patients diagnosed with non-ST segment elevation MI (NSTEMI). Total of 100 patients, 50 patients with NSTEMI and 50 healthy individuals, who had admitted to the cardiology outpatient clinic between January 2018 and January 2019 included in the study. Higher monocyte count, MHR, and TG/HDL-C ratios detected in patients with coronary artery disease in comparison to healthy individuals. Although monocyte count was higher in NSTEMI group, the difference was not statistically significant (p=0.101). There was an increase in the MHR NSTEMI group when compared against the control group (p

Relationship Between Gender, Age, Duration And Frequency Of Hemodialysis Therapy With The Creatinine Level reduction Of Pre And Post Hemodialysis Anny Thuraidah; Misbawati Misbawati; Nurlailah Nurlailah; Haitami Haitami
Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

The kidneys are organs of the body that function to regulate water balance in the body, the concentration of electrolytes in the blood and acid-base balance and secretion of waste material. If the kidney fails to function, the patient will need immediate treatment and even undergo hemodialysis (HD) therapy. A critical indicator in determining whether a person with impaired kidney function requires HD therapy is to know the creatinine level. The study aimed to ascertain the differences in creatinine levels pre and post HD also study the relationship between the age, gender, duration and frequency of HD therapy of respondents with the decrease of creatinine levels pre and post. Type of research detailed survey with a cross-sectional design. The sample was taken using a total sampling technique of 35 respondents from H BadaruddinKasimHospitalin Tanjung with a sample examination technique using the Jaffe method. The examination results of creatinine levels average for pre and post hemodialysis was11,36 and 5,58 mg/dl, which decreased 51%. The analysis statistically used Paired T-Test has a p-value = 0,000, indicating a significant difference for creatinine levels pre and post HD. Relationship between Age, Gender, Duration, and Frequency of HD Therapy with the decrease of creatinine levels pre and post HD was analyzed with Spearmen correlation and had p values more than 0,05. It means there was no relation between them. Conclusion there were significant differences in creatinine levels reduction pre and post HD while the relationship between the four of respondent characteristics to the magnitude of creatinine reduction show that there was no significant relationship.

Comparative study of diagnostic methods for determining the incidence of malaria parasite among children attending the State Specialist Hospital Ikare-Akoko, Ondo State, Nigeria Festus Abiose Olajubu; Esther Olubunmi Ojo
Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Malaria is an endemic disease in Sub-Saharan Africa, and it accounts for the death of more than 1million children annually. The objective of this study was to determine the incidence of malaria parasite among children between 2-15 years of age visiting State Specialist Hospital Ikare-Akoko, Ondo State, Nigeria, using Rapid diagnostic test kits and staining methods. The study does from July to December 2017. Two millimeters of venous blood samples were collected from each of the 300 enlisted children and stored in an anticoagulated specimen bottle. Thick and thin blood films prepared from the collected samples, stained and examined for the presence of malaria parasite use the Microscope. Three Rapid diagnostic test kits were also used. Two hundred and thirty-six (78.68%) of the 300 children investigated were found to have malaria parasite infection. Gender distribution showed that more male (45.67%) were infected than females (33.01%). Infections due to age distribution revealed that children within the ages of 2-5 years were more infected (39.3%) than children in other age range (6-10 years, 23.34% and 11-15 years, 17%). There was no statistical difference in the results obtained from the various techniques used (p

Serum Uric Acid Level in Unipolar and Bipolar Depression Gülçin Elboğa; Muhammet Berkay Özyürek; Zeynel Abidin Sayıner; Şengül Kocamer Şahin; Abdurrahman Altındağ
Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

The purinergic system plays a role in the regulation of mood, motor activity, cognitive function, sleep, and behavior. Purinergic mechanisms can also play a role in various neuropsychiatric diseases. The objective of this study is to assess whether uric acid levels in patients with unipolar and bipolar depression are different in comparison to healthy controls and to determine the clinical parameters that can be associated with the uric acid level. This retrospective study consisted of 31 patients with major depressive disorder and 31 patients with bipolar disorder depressive episode and 31 healthy control subjects. The mean serum uric acid levels were found as follows: major depression patient group 4.56 (± 1.53) mg/dL, bipolar depression patient group 5.38 (± 1.43) mg/dL and control group 4.86 (± 1.56) mg/dL. There was no significant difference between patients and the control groups in terms of serum uric acid levels (P=0.075). Serum uric acid levels do not differ significantly in bipolar and unipolar depression. Also, there was no difference between patients and control. Therefore, studying the uric acid metabolism in major depression and bipolar disorder depressive episode according to the symptom severity with larger sample groups is suggested.

Investigation of the Pathogenesis and Treatment Efficiency of Bevacizumab-Induced Hypertension in the Rat Model Mehmet Ali Balci; Musa Özgür Özyiğit; Volkan İpek; İlker Mustafa Kafa; Ender Kurt
Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Bevacizumab is known to reduce Vascular Endothelial Growth Factor (VEGF) to undetectable levels when used in conjunction with chemotherapy. Hypertension is a frequent adverse effect of bevacizumab, although its mechanism(s) remain unclear. In this study, our aim was to examine the pathogenesis of bevacizumab-induced hypertension and to investigate the treatment efficacy of valsartan. A total of 24 Wistar Albino female rats were included in the study. Rats were divided into three groups with 8 rats in each, as follows: The control group, bevacizumab group and bevacizumab + valsartan group. Blood pressure, blood urea nitrogen and serum creatinine levels were measured, urine samples were collected for 24 hours statistical analyses were performed using IBM SPSS 20 software pack. Nephrectomy specimens in bevacizumab and bevacizumab + valsartan groups exhibited varying degrees of renal injury. Although valsartan was able to reduce the bevacizumab-induced rise in blood pressure, it could not prevent the development of nephropathy. Conclusions these findings suggest that hypertension occurring secondary to bevacizumab treatment in rats may be associated with mechanisms involving renal injury.

The Comparison of Widal Slide Examination Results between Tubex TF on Febrile Observation Patients Over 3 Days Nosa Ika Cahyariza; Rofiatu Sholihah
Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Typhoid fever is systematic bacterial disease usually occurs and has a high mortality rate each year, a disease transmitted from person to person due to contamination of feces, food, and water. The cause is bacterium Salmonella enterica serovar Typhi (S. Typhi) which is a natural host and reservoir for human. The limitations of the diagnostic test led to the increasing mortality rate due to typhoid fever. Besides ensuring infection in individuals, accurate serological tests are needed to ascertain the actual burden of the disease. Serological tests which are usually carried out in Puskesmas and hospital are Widal and Tubex Tf examinations. This study aims to determine whether there are differences in Widal and Tubex TF serological examinations in febrile patients over three days non-typhoid so patients can immediately find out whether they have typhoid fever or not. This study used a laboratory exploration method by examining 24 samples using Widal TYDAL and TUBEX® TF IDL Biotech. As many as 24 samples were examined by widal with antisera O, H, AH, and BH. Twenty-four of the same samples analyzed by TUBEX® TF. Results comparison of diagnostic from both methods will be compared using Mc Nemar test with significance = 0.05. Based on the examination which had done, it showed the difference in the results of Widal slide and lg M Anti Salmonella (Tubex Tf) in patients with febrile observation over three days. So, it can conclude that Tubex Tf examinations were better that widal slide examination because Tubex Tf uses Salmonella typhi anti-O9 antigen which can distinguish these organisms from >99% other Salmonella bacteria serotypes so that Tubex Tf examination is more specific.

Prognostic Value of Neutrophil to Lymphocyte Ratio and Platelet to Lymphocyte Ratio in Lung Cancer Seda Beyhan Sagmen; Coskun Dogan; Sevda Comert; Nesrin Kiral; Ali Fidan; Elif Torun Parmaksiz
Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Numerous studies determined that the neutrophil/lymphocyte (NLR) and platelet/lymphocyte ratios (PLR) had prognostic value in several cancer types. This study aimed to evaluate the relationship between NLR and PLR values with the survival time of lung cancer patients. Patients diagnosed with lung cancer between January 2014 and December 2016, were retrospectively evaluated. Demographic characteristics, disease stages, laboratory parameters recorded, and the relationship of NLR and PLR values with the survival time and the disease stage evaluated. NLR and PLR were categorized into two groups. SPSS 17.0 software package was used for the statistical analysis. ROC analysis, Student T-test, Chi-square, and Mann-Whitney U test were used. Mean age of 62±8 years were included in the study. In the NSCLC group, the average NLR and PLR values were 4±3.35 and 194.6±144.4 respectively. Regarding the NSCLC group, the overall survival time was shorter in the subgroup with an NLR >3.43 (13.1 months) compared to the subgroup with an NLR ≤3.43 (24.3 months). The mean survival time was shorter in the group with a PLR > 136.9 compared to the group with a PLR ≤136.9 (15.9 and 24.6 months respectively). Subgroups consisting of survivors and non-survivors in the NSCLC group showed a statistically significant difference considering neutrophil and lymphocyte count, CRP, NLR, and PLR values (p

Microfilaria Detection on Giemsa Blood Smears using Real-Time PCR Paisal Paisal; Erli Haryati; Dwi Candra Arianti; Muhammad Rasyid Ridha; Annida Annida
Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Filariasis is an infectious disease caused by filarial worms, which found in many tropical and subtropical regions. In 2017, 12,677 cases of chronic filariasis were found in Indonesia, which 132 cases of them were from Central Kalimantan province. Data of Kapuas District Health Office shows 17 cases filariasis in 2015. Frequently, filariasis patients did not show any diseases symptoms, especially when the level of microfilariae in the blood is deficient. On the other hand, microscopic assay with Giemsa blood smears is still the gold standard to define filariasis. Thus, a false negative result may occur due to a low level of microfilariae in the blood. In this study, we develop a real-time PCR method, targeting the HhaI gene of filaria, to detect filarial worm from stored Giemsa blood taken from filariasis patients, in both dry and wet scraping methods. Our result shows that real-time PCR can detect Brugia malayi in all scraping samples, with Ct value from wet scraping sample tends to be higher than dry scraping. In conclusion, the real-time PCR method can be further used to define filariasis, especially in the condition when Giemsa smear blood cannot determine patient filariasis status.

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Ricko Dharmadi Utama

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Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

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Kota banjarmasin,

Kalimantan selatan

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ricko Dharmadi Utama

Contact Email apinaga53@gmail.com

Phone +6282256602225

Journal Mail Official editormltj@gmail.com

Editorial Address Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location Kota banjarmasin, Kalimantan selatan INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ricko Dharmadi Utama

Contact Email
apinaga53@gmail.com

Phone
+6282256602225

Journal Mail Official
editormltj@gmail.com

Editorial Address
Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location
Kota banjarmasin,

Kalimantan selatan

INDONESIA