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Agus Susanto
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R 108 Fakultas Peternakan Universitas Jenderal Soedirman, Jl. dr. Soeparno Utara No 60, Grendeng, Purwokerto Utara 53123 phone (0281) 638792 email: redaksijap@gmail.com - animalproduction@unsoed.ac.id
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Animal Production : Indonesian Journal of Animal Production
Animal Production is a peer-reviewed journal published by the Faculty of Animal Science, Jenderal Soedirman University in association with the Animal Scientist Society of Indonesia. The journal was established in 1999 and available online since May 4, 2011. Animal Production was initially published twice a year, then three times a year starting in 2005. The journal coverage focused on small scale livestock farming and technologies which includes the recent science development in animal production, including all aspects of nutrition, breeding, reproduction, post-harvest processing and socio-economics. All articles are available online. Full text available in pdf format and can be downloaded for free. Starting Vol 22 No 1 the papers have been and will be published in our new website (http://animalproduction.id), as the continuation of our previous but still active website (http://animalproduction.net).
Articles 11 Documents
Search results for , issue " Vol 11, No 3 (2009): September" : 11 Documents clear
Y Chromosome Microsatellites Variation in Bali Cattle (Bos sondaicus) Population Winaya, A; Muladno, Muladno; Gurnadi, RE; Saefuddin, A
ANIMAL PRODUCTION Vol 11, No 3 (2009): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Seven Y chromosome specific microsatellites were typed in a sample of 36 unrelated males from Bali cattle breed. Analysis variation of microsatellites marker in Bali cattle were determinate from PCR products by using 7 primer pairs that flanking microsatellites (INRA008, INRA057, INRA062, INRA124, INRA126, DYS 199, and INRA 189).  PCR products were separated by 10% polyacrylamide gel electrophoresis (PAGE), and silver staining method was used to detect allele polymorphism at each locus.  From two different geographical breed origin showed that Bali cattle from Bali island has higher heterozygous (h=0.33) level than Lombok island (h=0.30), but the number of allele was few (only two alleles) in all of locus. It is indicated that Bali cattle from two geographical origins were not significant in genetic variation. We know that Y chromosomal microsatellite in general has tend to specific allele in breed comparing to autosomal chromosome, because allele come from only male or Y sex chromosome and it contrary to autosomal chromosome where allele is contributed from male and female. FAO has specified that minimum four distinct alleles per locus for proficient judgment of genetic differences between breeds and this study we only used one breed, so it may not significantly to discriminate in Bali cattle population.  For next study we need more Y chromosomal microsatellite marker to discriminate more Indonesian breeds related to tracing the genetic potential and because males animal has roles in genetic spreading which can have an enormous impact on highly selected domestic animal populations. (Animal Production 11(3): 149-154 (2009) Key Words : Bali cattle , Y chromosome, microsatellite, allele

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