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R 108 Fakultas Peternakan Universitas Jenderal Soedirman, Jl. dr. Soeparno Utara No 60, Grendeng, Purwokerto Utara 53123 phone (0281) 638792 email: redaksijap@gmail.com - animalproduction@unsoed.ac.id
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Animal Production : Indonesian Journal of Animal Production
Published by Universitas Jenderal Soedirman
ISSN : 14112027     EISSN : 25415875     DOI : https://doi.org/10.20884/1.jap.2021.23.2.10
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary
Animal Production is a peer-reviewed journal published by the Faculty of Animal Science, Jenderal Soedirman University in association with the Animal Scientist Society of Indonesia. The journal was established in 1999 and available online since May 4, 2011. Animal Production was initially published twice a year, then three times a year starting in 2005. The journal coverage focused on small scale livestock farming and technologies which includes the recent science development in animal production, including all aspects of nutrition, breeding, reproduction, post-harvest processing and socio-economics. All articles are available online. Full text available in pdf format and can be downloaded for free. Starting Vol 22 No 1 the papers have been and will be published in our new website (http://animalproduction.id), as the continuation of our previous but still active website (http://animalproduction.net).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi
Articles 11 Documents
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Search results for , issue " Vol 9, No 2 (2007): May" : 11 Documents clear
Extraction of Collagen from Chicken Feet with Various Acidic Solutions and Soaking Time Prayitno, Prayitno
ANIMAL PRODUCTION Vol 9, No 2 (2007): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The objective of this research was to know the ability of various acidic solutions on dissolving collagen  chicken feet, with different soaked time.  Each acid 5 percent (v/v), collagen extraction was done by washing chicken feet and then cutted into small pieces and finally grinded.  Every 100 gram treatment was soaked in acetic acid (a1), citric acid (a2), lactic acid (a3) and hydrochloric acid (a4), for 12, 24 and 36 hours.  Precipitated collagen in the filtrate was 5 percent NaOH to reach the neutral pH (pH 7).  Collagen precipitate was separated by filtration usingfilter paper and then  rendement was calculated, HPLC was used to determin amino acid composition, and SDS-PAGE was use determin the type of collagen.  This experiment use factorial completely randomized design (CRD) 4 x 3 and three time replication.   Result showed that lactic acid has highest capability to dissolve collagen, while citric acid the lowest.  Combination of acid solution and soaking time had significant (P<0.01) effect on dissolving collagen of chicken feet.  Extracted collagen in all acid solution, hassame amino acid, composition but different in percentage of amino acid molecules.  Collagen type in treatment combination was the same, but for soaking time of 36 hours revealed some peptide band.  Lactic acid had highest capability of collagen extraction in chicken feet than citric acid, acetic acid and hydrochloric acid with soaking time of 12, 24 and 36 hours.  It was estimated that extracted collagen can be grouped to type I consisted of two chain of a1. (Animal Production 9(2): 99-104 (2007)  Key Words : Chicken feet, acids, soaking time, collagen

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