cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kab. gowa,
Sulawesi selatan
INDONESIA
Al-Kimia
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23022736     EISSN : -     DOI : -
Core Subject : Science,
Chemistry
Al-Kimia is a journal of chemistry that published by Department of Chemistry, Faculty of Science and Technology UIN Alauddin Makassar. The journal is published twice a year on January-June and July-December. This journal encompasses original research articles in all aspects of chemistry and related area. The journal, which was firstly published in July 2013, is a forum for communication of research results, review the results of research, methodologies and new approaches in research related to chemistry in Indonesia.
Arjuna Subject : -
Articles 11 Documents
 1   2 
Search results for , issue "Vol 6 No 2 (2018): December" : 11 Documents clear
Uric Acid Biosensor Based on Biofilm of L. plantarum using Screen-Printed Carbon Electrode Modified by Magnetite Dian Siska RF; Deden Saprudin; Dyah Iswantini; Novik Nurhidayat
Al-Kimia Vol 6 No 2 (2018): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v6i2.6300

Abstract

Biosensor based on biofilm of L. plantarum has been successfully done for determination of uric acid in human urine compared with colorimetric enzymatic produced relative error of less than 5%. L. plantarum has uricase activity to react with uric acid, to maintain the stability of bacteria forming themselves into biofilms. Magnetite is known to increase sensitivity of the biosensor. The combination of magnetite-polyethylene glycol (Fe3O4-PEG) was used to modify the surface of Screen-Printed Carbon Electrode modified (SPCE) and the resulting modified electrode (biofilm/Fe3O4/PEG/SPCE) displayed good electrocatalytic activity to the oxidation of UA. The composition of biofilms with optical density 1, magnetite 100 mgmL-1 and PEG 3% v / v were able to increase the current up to 48% in 4mM of UA. The biosensor with an optimum composition produced good linearity with a concentration range, limit of detection, limit of quantitation, sensitivity, and repeatability were found to be 0.1 - 4.3 mM, 70 µM,  234 µM, 25.392 µA mM-1, 2.38%, respectively. This biosensor stable up to 49 days of measurement with the remaining activity was 90.70% and selective for interference compounds such as salt, urea, glucose, ascorbic acid. This method has a good stability, sensitivity, and potential application in clinical analysis.

Page 2 of 2 | Total Record : 11

 1   2 

Filter by Year

2018 2018


Reset
Filter By Issues
All Issue Vol 13 No 1 (2025): JUNE Vol 12 No 2 (2024): DECEMBER Vol 12 No 1 (2024): JUNE Vol 11 No 2 (2023): DESEMBER Vol 11 No 1 (2023): JUNE Vol 10 No 2 (2022): DECEMBER Vol 10 No 1 (2022): JUNI Vol 9 No 2 (2021): DESEMBER Vol 9 No 1 (2021): JUNE Vol 8 No 2 (2020): DECEMBER Vol 8 No 1 (2020): JUNE Vol 7 No 2 (2019): DECEMBER Vol 7 No 1 (2019): JUNE Vol 6 No 2 (2018): December Vol 6 No 1 (2018): JUNE Vol 5 No 2 (2017): December Vol 5 No 1 (2017): June Vol 4 No 2 (2016): December Vol 4 No 1 (2016): June Vol 3 No 2 (2015): December Vol 3 No 1 (2015): June Vol 2 No 2 (2014): Desember Vol 2 No 1 (2014): June Vol 1 No 2 (2013): December Vol 1 No 1 (2013): June More Issue