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ALCHEMY Jurnal Penelitian Kimia
Published by Universitas Sebelas Maret
ISSN : 14124092     EISSN : 24434183     DOI : -
Core Subject : Science, Social, Engineering,
Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Materials Science & Nanotechnology
ALCHEMY Jurnal Penelitian Kimia is a chemistry journal published by Sebelas Maret University, Surakarta. ALCHEMY Jurnal Penelitian Kimia publishes original research articles or review articles in organic chemistry, inorganic chemistry, analytical chemistry, physical chemistry, biochemistry, and environmental chemistry.
Arjuna Subject : -
Articles 2 Documents
 1 
Search results for , issue "Vol 13, No 1 (2017): Maret" : 2 Documents clear
MOLECULAR WEIGHT PROFILE OF PROTEASE OF PINEAPPLE (Ananas comosus L.Merr) AND PAPAYA (Carica papaya L.) USING SDS-PAGE METHOD Rizky Arcinthya Rachmania; Priyo Wahyudi; Aniza Mutia Wardani; Dini Rohmatul Insani
ALCHEMY Jurnal Penelitian Kimia Vol 13, No 1 (2017): Maret
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.13.1.2540.52-65

Abstract

A group of protease enzymes such as papain and bromelain is able to decipher the molecular structure of the protein into amino acids which will be very useful in many fields, especially in food and pharmaceutical industries. The objective of this study to determine the molecular weight profile of enzyme bromelain from pineapple bark (Ananas comosus L. Merr) and papain (Carica papaya L.) from papaya latex with different varieties using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) method. The precipitation was performed with ammonium sulfate ((NH4)2SO4)) 60 % addition and dialysized using a cellophane tubing with a pore size of 12,000 Dalton. The molecular weight of enzyme solution were determined using SDS-PAGE. The analysis results of molecular weight of enzyme bromelain of Subang and Bogor varieties were not different and were about 30.654 kDa, as well as the molecular weight of enzyme papain and Sukma California varieties were also not different and were about 23.485 kDa. It can be concluded that the different varieties of fruit of pineapple and papaya had no effect on the molecular weight of enzyme papain and bromelain.
ZEOLITE-MODIFIED CARBON PASTE ELECTRODE FOR DETERMINATION OF COPPER USING ANODIC STRIPPING VOLTAMMETRY METHOD Irdhawati Irdhawati; Hery Suyanto; Putu Yani Andani
ALCHEMY Jurnal Penelitian Kimia Vol 13, No 1 (2017): Maret
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.13.1.1808.1-16

Abstract

In this research, the unmodified and modified carbon paste electrode with zeolite has been prepared, for determination of Cu(II) using anodic stripping voltammetry method. The parameters observed involved deposition time, scan rates, zeolite composition in carbon paste, and validation of working electrode. The optimum performance of carbon paste electrode modified with zeolite was applied for determination of Cu(II) in wastewater of the gong fabrication. The results of this research showed the optimum of deposition time and scan rates obtained at 410 s and 7.5 mV/s for unmodified carbon paste electrode, respectively. The optimum composition of zeolite-modifier is 20% from total graphite, with deposition time 380 s and scan rates 10 mV/s. The detection limit of the measurement of Cu(II) standard solution using unmodified carbon paste electrode is 46.13 ppb, is lower than unmodified carbon paste electrode, 99.93 ppb. Zeolite-modified carbon paste electrode has good precision and accuration. The concentration of Cu(II) in waste water of gong fabrication , using carbon paste electrode modified with zeolite is 93.54 ± 0.87 ppb.

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