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Sains Natural: Journal of Biology and Chemistry
Published by Universitas Nusa Bangsa
ISSN : 20863446     EISSN : 2621508X     DOI : https://doi.org/10.31938/jsn
Core Subject : Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry
Jurnal Sains Natural is a peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication with the objectives to explore the knowledge about natural sciences. This journal incorporates not only all branches of chemistry and biology, but also sub-disciplines like Biochemistry, Polymer, Agricultural chemistry, Environmental chemistry, etc.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 5 Documents
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Search results for , issue "Vol. 6 No. 2 (2016): Sains Natural" : 5 Documents clear
STATUS KUALITAS AIR TANAH DI KELURAHAN PEDURENAN, KECAMATAN MUSTIKAJAYA, BEKASI TIMUR Maulin Inggraini; Siti Nurfajriah; Pangeran Andareas
JURNAL SAINS NATURAL Vol. 6 No. 2 (2016): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.173 KB) | DOI: 10.31938/jsn.v6i2.161

Abstract

Status of  Ground Water Quality in The Village of Pedurenan, Mustikajaya District, East BekasiSanitation and poor hygiene practice and unsafe drinking water contributes to 88% of childhood deaths from diarrhea in worldwide. For children who survive often suffer from diarrhea contribute to nutritional problems, preventing children to be able to achieve their maximum potential. According to the Basic Health Research (Riskesdas) in 2013, the incidence and prevalence of diarrhea for all age groups in Indonesia is 3,5% and 7,0%. Respectively the incidence of diarrhea is closely associated with the quality of sanitation and groundwater used by society. This study aimed to determine the quality of water used for sanitation especially groundwater, in Sub Pedurenan, District Mustikajaya, East Bekasi. Results showed that groundwater quality inspection using the Most Probable Number (MPN) in RW 02, Village and District Pedurenan Mustikajaya, there were 4 samples were negative of coliform, and 25 coliform positive samples, with the highest number of 1100 cells / 100 mL. This indicates that ground water for sanitation and groundwater of sampling locations are generally contaminated by coliform bacteria.Keywords: groun water, coliform, diarrhea, water quality, MPN ABSTRAK Sanitasi dan perilaku kebersihan yang buruk serta air minum yang tidak aman berkontribusi terhadap 88% kematian anak akibat diare di seluruh dunia. Bagi anak-anak yang bertahan hidup, seringnya menderita diare berkontribusi terhadap masalah gizi, sehingga menghalangi anak-anak untuk dapat mencapai potensi maksimal mereka. Menurut hasil Riset Kesehatan Dasar (Riskesdas) tahun 2013, insiden dan prevalensi diare untuk seluruh kelompok umur di Indonesia adalah 3,5% dan 7,0%. Kejadian diare sangat erat kaitannya dengan kualitas sanitasi dan air tanah yang digunakan masyarakat. Penelitian ini bertujuan untuk mengetahui kualitas air yang digunakan untuk sanitasi serta air tanah khususnya di Kelurahan Pedurenan, Kecamatan Mustikajaya, Bekasi Timur. Hasil menunjukkah bahwa pemeriksaan kualitas air tanah menggunakan metode Most Probable Number (MPN) di RW 02 Kelurahan Pedurenan dan Kecamatan Mustikajaya, terdapat 4 sampel yang negatif coliform, dan 25 sampel positif coliform, dengan jumLah tertinggi 1100 sel/100 mL. Hal ini mengindikasikan bahwa air tanah untuk sanitasi dan air tanah di lokasi pengambilan sampel umumnya tercemar oleh bakteri coliform.Kata Kunci: Air tanah, coliform, diarrhea, Kualitas Air, MPN
PEMISAHAN ASAM AMINO DARI LIMBAH CAIR PABRIK KELAPA SAWIT DENGAN KROMATOGRAFI PENUKAR ION Indah Rahmi Sari; Ade Ayu Oksari; Irma Kresnawaty
JURNAL SAINS NATURAL Vol. 6 No. 2 (2016): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (628.747 KB) | DOI: 10.31938/jsn.v6i2.162

Abstract

Separation of Amino acid from Liquid waste of Oil palm Factory with Ion Exchange Chromatography Research on Separation of Amino Acid Liquid Waste mills with Ion Exchange Chromatography was carried out from October to November 2015. The results of  hydrolysis of 6 N HCl results showed  that the highest absorbance reading was obtained at a concentration of eluent of 0,2 and 0,6 M NaCl, while the results of the protease enzyme hydrolysis the highest absorbance reading at NaCl eluent 0,2 and 1 M. There was no significant difference in the results of separation by ion exchange chromatography, showed that the concentration of NaCl eluent is not very influential, so for subsequent analysis used only one concentration of the eluent. Results of linear regression obtained was equal to 0,9946, these results indicate that the series standard amino acid lysine has a value that is linear as it approaches 1. The amino acid levels obtained on the sample results LCPKS hydrolysis with 6 N HCl which was about 0 to 8.82 ppm and samples of the protease enzyme hydrolysis of about 0 to 4.31 ppm. Amino acid levels obtained were still far from the expected.Keywords: Amino Acid, Oil Palm, Liquid Waste, Ion Exchange Chromatography ABSTRAKPenelitian mengenai Pemisahan Asam Amino dari Limbah Cair Pabrik Kelapa Sawit dengan Kromatografi Penukar Ion telah dilaksanakan dari bulan Oktober sampai November 2015. Hasil hidrolisis HCl 6 N menunjukkan bahwa pembacaan absorbansi tertinggi diperoleh pada konsentrasi eluen NaCl 0,2 dan 0,6 M, sedangkan hasil hidrolisis enzim protease pembacaan absorbansi tertinggi pada eluen NaCl 0,2 dan 1 M. Tidak ada perbedaan yang signifikan pada hasil pemisahan dengan kromatografi penukar ion ini, menunjukkan bahwa konsentrasi eluen NaCl tidak terlalu berpengaruh, sehingga untuk analisis selanjutnya digunakan hanya satu konsentrasi eluen. Hasil regresi linear yang diperoleh yaitu sebesar 0,9946, hasil tersebut menunjukkan bahwa deret standar asam amino lisin mempunyai nilai yang linear karena mendekati 1. Kadar asam amino yang diperoleh pada sampel hasil hidrolisis LCPKS dengan HCl 6N yaitu sekitar 0 – 8,82 ppm dan sampel hasil hidrolisis enzim protease sekitar 0 – 4,31 ppm. Kadar asam amino yang diperoleh masih jauh dari yang diharapkan.Kata Kunci: Asam Amino, Minyak Kelapa Sawit, Limbah Cair, Kromatografi Penukar Ion
HIDROLISIS ASAM PADA TEPUNG PATI UBI JALAR PUTIH (Ipomoea batatas L.) DALAM PEMBUATAN GULA CAIR RTM Sutamihardja; Nia Yuliani; Hana Laelasari; Devy Susanty
JURNAL SAINS NATURAL Vol. 6 No. 2 (2016): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (494.161 KB) | DOI: 10.31938/jsn.v6i2.163

Abstract

Acid Hydrolysis on The Starch Flour of white sweet potato (Ipomoea batatas L.) in Making of Liquid SugarNational sugar needs for both direct consumption and for industrial needs will continue to increase as the population increases. According to Dirjenbun, in 2014 the national sugar demand reaches 5.7 million tons. Consisting of 2.8 million tons of white crystalline sugar for direct community consumption and 2.9 million tons of refined crystal sugar to meet industrial needs. White sweet potato can be used as raw material for making liquid glucose through hydrolysis process with acid (HCl). The preparation of liquid glucose consists of two stages: gelatinization stage and hydrolysis stage. Optimum hydrolysis was determined by variations in HCl concentration of 0.25; 0.5; and 0.75 N and time variations of 30, 60, and 90 minutes. The yield of sweet potato starch was 28.82% and the highest yield of hydrolysis result of white sweet potato starch was 94.07% at acid concentration 0.75 N with hydrolysis time 90 minutes.Keywords: white sweet potato, Flour of white sweet potato, liquid sugar, acid hydrolysisABSTRAKKebutuhan gula nasional baik untuk konsumsi langsung maupun untuk kebutuhan industri akan terus meningkat sejalan dengan meningkatnya jumlah penduduk. Menurut Dirjenbun, pada tahun 2014 kebutuhan gula nasional mencapai 5,7 juta ton yang terdiri dari 2,8 juta ton gula kristal putih untuk konsumsi masyarakat langsung dan 2,9 juta ton gula kristal rafinasi untuk memenuhi kebutuhan industri. Ubi jalar putih dapat dijadikan bahan baku pembuatan glukosa cair melalui proses hidrolisis dengan asam (HCl). Pembuatan glukosa cair terdiri dari dua tahap yaitu tahap gelatinisasi dan tahap hidrolisis. Hidrolisis optimum ditentukan dengan variasi konsentrasi HCl yaitu 0,25; 0,5; dan 0,75 N dan variasi waktu 30, 60, dan 90 menit. Rendemen pati ubi jalar didapatkan sebesar 28,82% dan rendemen glukosa tertinggi hasil hidrolisis pati ubi jalar putih sebesar 94,07% pada konsentrasi asam 0,75 N dengan waktu hidrolisis 90 menit.Kata kunci: Ubi jalar putih, Tepung Pati Ubi Jalar Putih, Gula cair, hidrolisis asam
UJI KANDUNGAN HIDROKSIMETILFURFURAL (HMF) SEBAGAI PARAMETER KUALITAS MADU Audi Rizki Koesprimadisari; Dian Arrisujaya; Resty Syafdaningsih
JURNAL SAINS NATURAL Vol. 6 No. 2 (2016): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1051.198 KB) | DOI: 10.31938/jsn.v6i2.159

Abstract

Hydroxymethylfurfural Content Test as Parameter Quality of Honey            Determination hydroxymethylfurfural (HMF) levels in honey had been done refer to method in SNI 3545:2013.  Average of the HMF levels is 64,72 mg/kg whereas SNI decided maximal limit was 50 mg/kg so honey quality is not good enough.  To support testing result, calculation of reapeatabily with relative standard deviation (RSD) Horwitz and accuracy with % recovery was done. The result of reapeatabilty showed that analyst reapeatability was good because the relative standard deviation less than 2/3 RSD Horwitz.  The result of accuracy was good because both treatment gave recovery 89,28 % and 106,43 %, which in the range of acceptance recovery test for analite concentration about 50 mg/kg.Keywords : HMF, Hydroxymethylfurfural, Honey, Quality of Honey , Reapeatability, Accuracy  ABSTRAKPenentuan kadar hidroksimetilfurfural (HMF) pada madu telah dilakukan dengan metode yang mengacu pada SNI 3545:2013. Rata-rata hasil kandungan hidroksimetilfurfural adalah 64,72 mg/kg sedangkan batas maksimal yang ditetapkan SNI adalah 50 mg/kg sehingga kualitas contoh madu tersebut kurang baik.  Untuk menunjang hasil pengujian, maka dilakukan perhitungan repeatabilitas dengan menggunakan Standar Baku Relatif (SBR) Horwitz dan perhitungan akurasi menggunakan % perolehan kembali. Hasil perhitungan repeatabilitas menunjukkan bahwa repeatabilitas analisis HMF baik karena nilai simpangan baku lebih kecil dari 2/3 SBR Horwitz.  Perhitungan akurasi pun menunjukan hasil yang baik karena kedua perlakuan memberi hasil perolehan kembali sebesar 89,28 % dan 106,43 %, yang masuk kedalam batas keberterimaan uji perolehan kembali untuk konsentrasi analit dalam contoh sekitar 50 mg/kg.Kata Kunci : HMF, Hidroksimetilfurfural, Madu, Kualitas Madu, Reapitabilitas, Akurasi
OPTIMASI PENAMBAHAN MADU SEBAGAI ZAT ANTI BAKTERI Staphylococcus aureus, PADA PRODUK SABUN MANDI CAIR Alvera Raisa; Srikandi Srikandi; Ricson P. Hutagaol
JURNAL SAINS NATURAL Vol. 6 No. 2 (2016): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (837.148 KB) | DOI: 10.31938/jsn.v6i2.160

Abstract

Optimization of the Addition of Honey as an agent of an Anti bacterial Agent Staphylococcus aureus in Production of Shower liquid soapThe study was begun with making the basic shower liquid soap, then aditing the honey with a variety of different concentrations. In this study, the addition of honey were 0%; 2,5%; 5%; 7,5%; 10%; 12,5% and 15%. After that tested the effectiveness of antibacterial agent microbiology. Then analyzing physical and chemical properties of liquid soap in accordance with SNI 06-4085-1996. The parameters tested were pH, viscosity, and density, test quantity foam, and test preferences. Optimal concentration of the addition of honey in a liquid bath soap to be able to inhibit the growth of Staphylococcus aureus are at the level of 10%. When compared with Triclosan soap, shower liquid soap with the addition of honey 5% could compete with antibacterial properties of triclosan soap using a concentration of 0.3%. The addition of honey with various concentrations of honey affect the physical and chemical properties of liquid soap such as pH, viscosity, density, and the amount of foam.Keywords: Liquid Soap, Honey and bacteria Staphylococcus aureus   ABSTRAKPenelitian dimulai dengan melakukan pembuatan dasar sabun mandi cair, kemudian dilakukan penambahan madu dengan berbagai konsentrasi berbeda. Dalam penelitian ini dilakukan penambahan madu sampai lebih dari 5% yaitu 0%; 2,5%; 5%; 7,5%; 10%; 12,5% dan 15%. Setelah itu dilakukan uji efektifitas dari zat antibakteri secara mikrobiologi. Kemudian dilakukan analisis terhadap sifat fisika dan kimia sabun mandi cair sesuai dengan SNI 06-4085-1996.  Parameter yang diuji adalah pH, viskositas, dan berat jenis, uji banyak busa, dan uji kesukaan. Konsentrasi optimal penambahan madu pada sabun mandi cair untuk bisa menghambat pertumbuhan bakteri Staphylococcus aureus adalah pada taraf 10%. Bila dibandingkan dengan sabun Triclosan, sabun mandi cair dengan penambahan madu 5% dapat menyaingi sifat antibakteri dari sabun yang menggunakan Triclosan dengan konsentrasi 0,3%. Penambahan madu dengan berbagai konsentrasi berpengaruh kepada sifat fisika dan kimia dari sabun mandi cair seperti pH, viskositas, berat jenis, serta jumlah busa.Kata kunci :  Sabun Mandi Cair, Madu dan Bakteri Staphylococcus aureus

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