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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 38 Documents
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Search results for , issue "Vol 12, No 1 (2020): April 2020" : 38 Documents clear
The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation Raudhah Mahfudhah; Kartini Eriani; Zainal Abidin Muchlisin
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22353

Abstract

The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds.
Microbiological Quality and Proximate Composition of Bakasang Laor, a Traditional Fermented Fishery Product in Maluku Ferymon Mahulette; Tri Santi Kurnia
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.23356

Abstract

Bakasang laor is one of the traditional fermented fishery products in Maluku, Indonesia. There are two types of  bakasang laor, i.e. with and without vinegar. The microbiological research of bakasang processed use laor as raw material  has never been done before. The research aimed to analyze the microbiological quality and proximate composition of two types of bakasang laor.  The proximate composition can determine the nutritional value of  this product for consumption by the community. The sample of bakasang laor was taken from traditional producer in Latuhalat village, Ambon. The total number of halotolerant and coliform bacteria  in bakasang laor without vinegar were 1.7x106 CFU/g and 4.1x106 CFU/g, respectively, while the total of lactic acid bacteria in bakasang laor with vinegar was 6.0x106 CFU/g.  The carbohydrate, protein, fat and ash contents of bakasang laor with vinegar was higher than bakasang laor without vinegar. The carbohydrate, protein and fat contents of bakasang laor with vinegar were 19.64 %, 16.23 %, and 2.42 %, respectively. Generally, microbiological quality and proximate composition  of  bakasang laor with vinegar was better than bakasang laor without  vinegar. The bacteria that play a role in fermentation can be used as a starter in the fermentation of various food products.  The results of this research to improve the quality of this fermentation product in the future.
Traditional Knowledge of Banyumas Community about Mesua ferrea as the Identity Plant Enni Suwarsi Rahayu; Erni Suharini; Siti Fathonah; Aisirotul Maisah
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.23834

Abstract

The population of Mesua ferrea L. in Banyumas Regency, Central of Java Province is very low. There are some factors affecting the quantity of plant population, particularly traditional knowledge of local community about the plant. The aim of this study was to describe the knowledge of Banyumas community about the existence, economic value, usefulness, and propagation technique of M. ferrea. The study was done by field survey on a total of 146 residents from 10 districts which were determined by proportionally stratified random sampling. Data obtained from questionnaires and interviews were analyzed by percentage descriptive. The results revealed that only 9% of respondents were familiar with the M. ferrea and knew its status as plant identity of Banyumas Regency. Most of the respondents did not understand the economic value, only 8% of respondents understood the usefulness, 2% of respondents had utilized the timber as craft material, and 3% understood the propagation technique. The utilization was very low, equal to 14.45% compared to the potential usefulness. Most of the respondents (98%) thought that conservation efforts should be undertaken. This study found that the traditional knowledge of M. ferrea in Banyumas was low. This result should be used as a basic information for developing conservation program of M. ferrea.  
Induction and Growth Kinetics Callus of Tomato (Solanum lycopersicum) Arkan Setiaji; RR Rifka Annisa; Rumiyati Rumiyati; Endang Semiarti
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.21704

Abstract

Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ‘Permata’) for optimum callus induction. Seeds were grown on ¼ MS medium for 10-15 days. Hypocotyl was excised and cultured on MS medium + 2 mg/l 2,4-D for 15 days as the explants for callus induction. Callus was transferred to MS medium with 8 variations of PGRs including the combination of BAP + NAA, and 2,4-D. Both fresh and dry weight was measured every 5 days over 60 days to establish the growth kinetics and growth efficiency of callus. Anatomic characters of calli were examined through paraffin-embedded method. The result showed of MS medium supplemented with 2.0 mg/l NAA and 0.2 mg/l BAP is optimum for tomato callus induction, based on highest number of the absolute growth rate on fresh weight (73.77% per day), dry weight (3.84% per day), and callus initiation time (5.56 days) achieved by the medium. Cells in the ground tissue of tomato hypocotyl are competent to be dedifferentiated into a callus. This research results were expected to find out suitable methods for tomato callus production in preparation for skincare uses.
Frontmatter (Cover and Table of Contents) Cover Daftar Isi
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.24288

Abstract

Tyrosinase Inhibition, Antiglycation, and Antioxidant Activity of Xylocarpus granatum Irmanida Batubara; Maily Mustofa; Wulan Tri Wahyuni; Kilala Tilaar; Waras Nurcholis; Fransiska Devy Junardy; Yogo Suro Priyadi; Erna Subroto; Saat Egra; Nevianti Zamany
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22676

Abstract

Xylocarpus granatum is mangrove plant that traditionally used as face powder in Central Sulawesi, Indonesia which related to antioxidant, antiglycation and tyrosinase inhibition activities. This study aimed to evaluate the potency of X. granatum as a tyrosinase inhibitor, antiglycation, and antioxidant. The leaves, stem, stem bark, fruit flesh, fruit peel, and kernel of X. granatum were extracted using ethanol then their tyrosinase inhibition, antiglycation, and antioxidant were evaluated. Tyrosinase inhibition activity was evaluated using in vitro assay with L-tyrosine and L-DOPA as the substrate of monophenolase and diphenolase. Antiglycation activity was studied by measuring the excitation and emission fluorescence from glucose and fructose reaction with Bovine Serum Albumin. Antioxidant activity was measured using the DPPH radical scavenging assay. The result showed that the ethanolic extract of fruit flesh has higher potency as tyrosinase inhibitor (IC50 of 393.8 mg/L and IC50 of 448 mg/L, respectively for monophenolase and diphenolase). Antiglycation assay showed that the ethanolic extract of stem bark provides the strongest antiglycation activity with an IC50 of 118.1 mg/L. Meanwhile, fruit peel provides the strongest antioxidant activity with an IC50 of 5.5 mg/L. Fractionation of ethanolic extracts of each part of X. granatum tree yield fractions with lower bioactivity compared to the crude extract. Moreover, stem extract and fractions from two different locations (Tarakan and Kendari) tend to have different bioactivities strengths.  The stem part of X granatum could be developed as new raw material of cosmetic product in Indonesia, while ethanol as the solvent for extraction, and the different bioactivity of stem extract from different location can be the consideration for the industry to standardize the extract prior to production of final product.
Production of Conidia by Entomopathogenic Fungi and Their Pathogenicity Against Coptotermes sp. Deni Zulfiana; Apriwi Zulfitri; Anis Sri Lestari; Ni Putu Ratna Ayu Krishanti; Dita Meisyara
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22435

Abstract

Entomopathogenic fungi have the potential to infect most arthropods including termites which are economically important major insects pest of wood, wood products and building structures. However, the application of this fungus in the field has not shown satisfactory results yet, one of which is constrained in mass production of conidia. The purpose of this study was to evaluate 16 types of biodegradable products and waste as substrates for mass production of conidia using solid state fermentation method and two types of inoculum namely solid and liquid inoculum. Toxicity tests were carried out on subterranean termites (Coptotermes sp.) based on JIS K 1571, 2010. The parameters observed were the number and dry weight of the conidia produced, conidial viability, nutrient content of the substrate, and percentage of termite mortality. The results showed that rice, sorghum and corn were the best media for the growth of entomopathogenic fungi based on the number of conidia and dry weight of the conidia produced. Metarhizium sp. T4.B23 produced the highest number of conidia, 1.12 x 1011 conidia/100 g substrate and yielded 180.9 ± 0.623 g dry conidia/kg of rice; followed by Metarhizium sp. B2.2 grown on sorghum that resulted in 1.11 x 1010 conidia/100 g substrates and 8 ± 0.570 g /kg sorghum; and B. bassiana produced 8.3 x 109 conidia/100 g substrate and 31.24 ± 0.407 gr/ kg sorghum. Metarhizium sp. B2.2 showed the highest toxicity to termites with 100% mortality was observed within the second day of testing. Therefore the conidia of Metahizium sp. B2.2 is potential to be developed as a biopesticide using rice or sorghum substrate as a carrier.
Molecular Barcoding Reveal the Existence of Mole Crabs Emerita emeritus in North Coast of Central Java Dian Bhagawati; Elly Tuti Winarni; Agus Nuryanto
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.20497

Abstract

The existence of mole crabs had been reported from the southern coast of Central Java. However, no mole crab data was available from the northern coast Central Java.  This study aimed to figure out the existence of mole crabs in the northern coast of Central Java as revealed from the cytochrome c oxidase 1 (COI) barcoding. Mole crabs samples collected in Sendang Sikucing Beach Kendal. Taxonomic status of the samples was inferred from sequences similarity test using basic local alignment search tool to conspecific sequence deposited in GenBank. Pairwise genetic distances were calculated based on the Kimura 2-parameter model. A phylogenetic tree had reconstructed in molecular evolution genetic analysis (MEGA) software based on neighbor-joining algorithm. Branching reliability was obtained from 1000 bootstraps replication while branching polarity was obtained from the out-group comparison. The mole crab samples from the northern coast of Central Java had unambiguously identified as Emerita emeritus based on high sequences similarities (98.27%-99.70%), low genetic distances (0.002-0.005), and their monophyly with Emerita emeritus (KR047035) in GenBank. Pairwise analysis among each possibility pair of samples had genetic distances ranged between 0.000 and 0.005, indicated that all sample belong to single species. The data provide the first record of the existence of Emerita emeritus in the northern coast of Central Java. Our data on the existence of E. emeritus in the northern coast of Central Java is among essentials information as a scientific basis in creating policy for the management of sustainable use of the mole crabs in the areas.

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