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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 54 Documents
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Search results for , issue "Vol 12, No 3 (2020): December 2020" : 54 Documents clear
Blood Hematological and Biochemical Parameters of Osteochilus vittatus with Spirulina platensis Supplementation in Biofloc System Muhammad Azharul Rijal; Susanto Susanto; Sorta Basar Ida Simanjuntak; Hernayanti Hernayanti; Purnama Sukardi
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.20936

Abstract

Spirulina platensis is a microalgae that contains nutrients such us iron and phycocianin higher than other microalgae. Research on suplementation of S. platensis in Osteochilus vittatus cultured in biofloc system has never been done. The aim of this study was to determine the blood hematological and biochemical parameters of O. vittatus fed with S. platensis supplementation and maintained in biofloc system. The study was conducted experimentally with Completely Randomized Design, four treatments and five replications. O. vittatus were fed with S. platensis level 0, 2, 4, and 6 g.kg-1 for 56 days. Blood hematological and biochemical parameters were measured on days 0 and 56. Values of those parameters were then analyzed using ANOVA with a confidence level of 95%. The result showed that the highest red blood cells, white blood cells, and hemoglobin counts were 1.57x106­­­­ cell.mm-3, 2.37x105 cell.mm-3, and 6.77 g.dl-1 respectivley, while the highest hematocrit value was 17.5 %. The highest total protein, albumin, and globulin in blood were 7.96 g.dL-1, 4.31 g.dL-1, and 3.79 g.dL-1 respectively, and the best for ratio A/G was 1.14. S. platensis suplementation level of 4g.kg-1 feed was the most optimum level (P0.05). Cultivation of fish with S. platensis supplementation in feed can increase fish health indicated by the hematological and biochemical parameters of the blood and the fish maintenance in biofloc systems can improve water quality. The benefit of this research is to make a good condition for cultivation and efficiently used not only water but also feed.
Larvicidal Activity of Brugmansia candida against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae) Setiawan Khoirul Himmi; Didi Tarmadi; Dita Meisyara; Anugerah Fajar; Titik Kartika; Ikhsan Guswenrivo; Sulaeman Yusuf
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24634

Abstract

Mosquitoes are well known as vectors of hazardous diseases for human. Plant extracts can be used as an alternative for larval control due to they are a rich source of bioactive chemicals and safe for the environment. The present study investigated the larvicidal activity of crude extracts derived from leaf and flower of Brugmansia candida against the second larval instar of Aedes aegypti and Culex quinquefasciatus. The larval mortality was observed at 24 and 48 h exposure of both leaf and flower extracts, at the concentration of 100, 250, 500, and 1000 ppm. The 24 h exposure of both extracts at the concentration of 500 and 1000 ppm resulted in larval mortality rates were significantly lower than those of 48 h exposure. However, the mortality rate was not significantly different at the lower concentrations of crude extracts. The results also suggested that there was no significant difference in the larvicidal effect between leaf and flower extracts at 24 and 48 h exposure for all concentrations. The LC50 values at 48 h exposure for leaf extract were 789 and 791 ppm for Ae. aegypti and C. quinquefasciatus, respectively, whereas for flower extract were 772 and 780 ppm for Ae. aegypti and C. quinquefasciatus, respectively. Overall, B. candida showed larvicidal activity against Ae. aegypti and C. quinquefasciatus. This research contributes to new finding regarding the larvicidal activity of B. candida. This finding also supports the next study to develop B. candida as an alternative source for larval control agent.
Identification of Gamma-Amino Butyric Acid Isolates Lactic Acid Bacteria Results from The Isolation of Rusip S Feta Avila Prayitno; Agung Suprihadi; Endang Kusdiyantini
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24995

Abstract

Gamma-aminobutyric acid (GABA) is a non-protein amino acid produced by glutamate decarboxylation by the enzyme glutamate decarboxylase and it is widely distributed in plants, animals and microorganisms. GABA-producing microorganisms including Lactic Acid Bacteria (LAB) which play a role in the fermentation process of food such as rusip. Rusip is a preserved food of marine fish made from raw anchovy of Bangka-Belitung. The purpose of this study was to obtain LAB isolates through the process of isolation from rusip and identify the presence of GABA. Isolation of LAB was carried out by the pour method on MRS agar and NA medium. The growing colonies were then characterized based on observations of colony morphology, Gram staining, catalase test, motility test, and fermentation type test. LAB isolates obtained were selected to obtain isolates which are capable to produce GABA using the Thin Layer Chromatography (TLC) method. The results of isolation from rusip and inoculation on MRS agar medium obtained, three LAB isolates namely RSP-A1, RSP-A2, and RSP-A3. Their characteristics are Gram positive bacteria, negative catalase test, negative motility test, and belong to homofermentative and heterofermentative bacteria groups. They were also able to produce GABA. Research on GABA from isolates isolated from rusip is a new thing in the world of GABA research. This research provides information that is beneficial to produce GABA easily, cheaply, and efficiently.
In Situ Bioremediation Strategies for the Recovery of Mercury-contaminated Land in Abandoned Traditional Gold Mines in Indonesia Winardi Winardi; Eko Haryono; Sudrajat Sudrajat; Endang Sutariningsih Soetarto
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25229

Abstract

Traditional gold mining activities release mercury into the environment, creating a major concern for the Indonesian governments today. In situ bioremediation, which draws on the activities of indigenous soil bacteria for the recovery of mercury-contaminated land, has never been conducted intensively in the country. This research set out to determine the most efficient in situ bioremediation strategy for this purpose. It took place in Mandor Village, Landak Regency, Kalimantan Barat-Indonesia. During the experiment, four groups of sampling plots were made into triplicate and given various treatments: a. nutrient addition, b. aeration, c. pH neutralization, and d. without nutrient addition and aeration as a control. pH neutralization was conducted in all sampling plots by adding lime until soil pH of ±7 was achieved. The experiment was performed during both rainy and dry seasons to determine the influence of seasonal weather. Total mercury levels of each plot were measured on day 0, 30, 60, 90, and 120, and the effects of treatments and time on mercury depletion were analyzed by two-way ANOVA (P0.05), followed by a post hoc test to identify the best treatment and optimum time for in situ bioremediation. The results showed that the best time to conduct this bioremediation was in the rainy season by applying nutrient addition and aeration for 90 days on soils with neutral pH; these stimulations could remove ±89.6% of the mercury. This bioremediation technique is a novel technological approach in land recovery that local governments can adopt to restore soils contaminated with mercury from traditional gold mining.  

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